In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates

Danjuma Lawal; Bobai Mathew; Sani Muhammad Nura

doi:doi:10.11648/j.jb.20220601.12

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In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates

Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura

Published in Journal of Biomaterials (Volume 6, Issue 1)

Received: 11 June 2022 Accepted: 18 July 2022 Published: 17 August 2022

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Abstract

Most available antimicrobials are now ineffective and the whole world healthcare system is currently under threat of antimicrobial resistant infections. Consequently, study of plant bioactive compounds with potentials infectious diseases therapeutic values is of significant risen interest with biologically synthesized plant extracts derived silver nanoparticles on the greater focus. This research was aimed at determining the in vitro antimicrobial activity of silver nanoparticles synthesized from extracts of Terminalia avicennioides on antibiotic resistant Pseudomonas aeruginosa isolates from wounds. Standard phenotypic and genotypic techniques were used for the Isolation and identification of Pseudomonas aeruginosa isolates. Selected antibiotics, Terminalia avicennioides extracts and the extracts derived silver nanoparticles antimicrobial activities on the antibiotic resistant Pseudomonas aeruginosa were determined using standard tests methods. Findings showed the isolates to be resistant to 18.18% - 100% of the antibiotics used, but 100% sensitive to imipenem. Analysis of the plant extracts for bioactive compounds showed the presence of tannins, alkaloids, flavonoids, cardiac glycosides, phenols, saponins and terpenoids. Antimicrobial profile of Terminalia avicennioides extracts on the antibiotic resistant Pseudomonas aeruginosa isolates showed zones of growth inhibition ranged from 10.04±9.39 – 18.08±10.62 mm with no significant difference (P > 0.05), minimum inhibitory concentration ranged from 60.000+65.8281 -40.000 + 21.0821 mg/ml with no significant difference (p < 0.05), and minimum bactericidal concentration ranged from 100.00 ± 89.4427 – 63.6364 ± 50.4525 mg/ml with no significant difference (p > 0.05). The antimicrobial activity of the biologically synthesized silver nanoparticles on the antibiotic resistant Pseudomonas aeruginosa showed zone of growth inhibition ranged from 28.00 ± 13.51 – 53.00 ± 76.97 mm with no significant difference (p > 0.05). Terminalia avicennioides extracts and silver nanoparticles antimicrobial activity showed significant difference (p > 0.05). In comparison, the silver nanoparticles zones of growth inhibition was larger (28.39 ± 2.98 mm) than that of the extracts (16.83 ± 12.70 mm). This inferred that the synthesized silver nanoparticles possess potential of being used as a good chemotherapeutic agent for wound infections.

Published in	Journal of Biomaterials (Volume 6, Issue 1)
DOI	10.11648/j.jb.20220601.12
Page(s)	5-19
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pseudomonas aeruginosa, Antibiotic Resistant, Nanoparticles, Wound, Antimicrobial, Terminalia avicennioides

References

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Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura. (2022). In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. Journal of Biomaterials, 6(1), 5-19. https://doi.org/10.11648/j.jb.20220601.12

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ACS Style

Danjuma Lawal; Bobai Mathew; Sani Muhammad Nura. In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. J. Biomater. 2022, 6(1), 5-19. doi: 10.11648/j.jb.20220601.12

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AMA Style

Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura. In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. J Biomater. 2022;6(1):5-19. doi: 10.11648/j.jb.20220601.12

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@article{10.11648/j.jb.20220601.12,
  author = {Danjuma Lawal and Bobai Mathew and Sani Muhammad Nura},
  title = {In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates},
  journal = {Journal of Biomaterials},
  volume = {6},
  number = {1},
  pages = {5-19},
  doi = {10.11648/j.jb.20220601.12},
  url = {https://doi.org/10.11648/j.jb.20220601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20220601.12},
  abstract = {Most available antimicrobials are now ineffective and the whole world healthcare system is currently under threat of antimicrobial resistant infections. Consequently, study of plant bioactive compounds with potentials infectious diseases therapeutic values is of significant risen interest with biologically synthesized plant extracts derived silver nanoparticles on the greater focus. This research was aimed at determining the in vitro antimicrobial activity of silver nanoparticles synthesized from extracts of Terminalia avicennioides on antibiotic resistant Pseudomonas aeruginosa isolates from wounds. Standard phenotypic and genotypic techniques were used for the Isolation and identification of Pseudomonas aeruginosa isolates. Selected antibiotics, Terminalia avicennioides extracts and the extracts derived silver nanoparticles antimicrobial activities on the antibiotic resistant Pseudomonas aeruginosa were determined using standard tests methods. Findings showed the isolates to be resistant to 18.18% - 100% of the antibiotics used, but 100% sensitive to imipenem. Analysis of the plant extracts for bioactive compounds showed the presence of tannins, alkaloids, flavonoids, cardiac glycosides, phenols, saponins and terpenoids. Antimicrobial profile of Terminalia avicennioides extracts on the antibiotic resistant Pseudomonas aeruginosa isolates showed zones of growth inhibition ranged from 10.04±9.39 – 18.08±10.62 mm with no significant difference (P > 0.05), minimum inhibitory concentration ranged from 60.000+65.8281 -40.000 + 21.0821 mg/ml with no significant difference (p  0.05). The antimicrobial activity of the biologically synthesized silver nanoparticles on the antibiotic resistant Pseudomonas aeruginosa showed zone of growth inhibition ranged from 28.00 ± 13.51 – 53.00 ± 76.97 mm with no significant difference (p > 0.05). Terminalia avicennioides extracts and silver nanoparticles antimicrobial activity showed significant difference (p > 0.05). In comparison, the silver nanoparticles zones of growth inhibition was larger (28.39 ± 2.98 mm) than that of the extracts (16.83 ± 12.70 mm). This inferred that the synthesized silver nanoparticles possess potential of being used as a good chemotherapeutic agent for wound infections.},
 year = {2022}
}

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TY  - JOUR
T1  - In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates
AU  - Danjuma Lawal
AU  - Bobai Mathew
AU  - Sani Muhammad Nura
Y1  - 2022/08/17
PY  - 2022
N1  - https://doi.org/10.11648/j.jb.20220601.12
DO  - 10.11648/j.jb.20220601.12
T2  - Journal of Biomaterials
JF  - Journal of Biomaterials
JO  - Journal of Biomaterials
SP  - 5
EP  - 19
PB  - Science Publishing Group
SN  - 2640-2629
UR  - https://doi.org/10.11648/j.jb.20220601.12
AB  - Most available antimicrobials are now ineffective and the whole world healthcare system is currently under threat of antimicrobial resistant infections. Consequently, study of plant bioactive compounds with potentials infectious diseases therapeutic values is of significant risen interest with biologically synthesized plant extracts derived silver nanoparticles on the greater focus. This research was aimed at determining the in vitro antimicrobial activity of silver nanoparticles synthesized from extracts of Terminalia avicennioides on antibiotic resistant Pseudomonas aeruginosa isolates from wounds. Standard phenotypic and genotypic techniques were used for the Isolation and identification of Pseudomonas aeruginosa isolates. Selected antibiotics, Terminalia avicennioides extracts and the extracts derived silver nanoparticles antimicrobial activities on the antibiotic resistant Pseudomonas aeruginosa were determined using standard tests methods. Findings showed the isolates to be resistant to 18.18% - 100% of the antibiotics used, but 100% sensitive to imipenem. Analysis of the plant extracts for bioactive compounds showed the presence of tannins, alkaloids, flavonoids, cardiac glycosides, phenols, saponins and terpenoids. Antimicrobial profile of Terminalia avicennioides extracts on the antibiotic resistant Pseudomonas aeruginosa isolates showed zones of growth inhibition ranged from 10.04±9.39 – 18.08±10.62 mm with no significant difference (P > 0.05), minimum inhibitory concentration ranged from 60.000+65.8281 -40.000 + 21.0821 mg/ml with no significant difference (p  0.05). The antimicrobial activity of the biologically synthesized silver nanoparticles on the antibiotic resistant Pseudomonas aeruginosa showed zone of growth inhibition ranged from 28.00 ± 13.51 – 53.00 ± 76.97 mm with no significant difference (p > 0.05). Terminalia avicennioides extracts and silver nanoparticles antimicrobial activity showed significant difference (p > 0.05). In comparison, the silver nanoparticles zones of growth inhibition was larger (28.39 ± 2.98 mm) than that of the extracts (16.83 ± 12.70 mm). This inferred that the synthesized silver nanoparticles possess potential of being used as a good chemotherapeutic agent for wound infections.
VL  - 6
IS  - 1
ER  -

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Author Information

Danjuma Lawal

Department of Microbiology and Biotechnology, Federal University Dutse, Jigawa, Nigeria
Bobai Mathew

Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria
Sani Muhammad Nura

Department of Microbiology and Biotechnology, Federal University Dutse, Jigawa, Nigeria

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Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura. (2022). In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. Journal of Biomaterials, 6(1), 5-19. https://doi.org/10.11648/j.jb.20220601.12

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ACS Style

Danjuma Lawal; Bobai Mathew; Sani Muhammad Nura. In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. J. Biomater. 2022, 6(1), 5-19. doi: 10.11648/j.jb.20220601.12

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AMA Style

Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura. In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. J Biomater. 2022;6(1):5-19. doi: 10.11648/j.jb.20220601.12

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@article{10.11648/j.jb.20220601.12,
  author = {Danjuma Lawal and Bobai Mathew and Sani Muhammad Nura},
  title = {In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates},
  journal = {Journal of Biomaterials},
  volume = {6},
  number = {1},
  pages = {5-19},
  doi = {10.11648/j.jb.20220601.12},
  url = {https://doi.org/10.11648/j.jb.20220601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20220601.12},
  abstract = {Most available antimicrobials are now ineffective and the whole world healthcare system is currently under threat of antimicrobial resistant infections. Consequently, study of plant bioactive compounds with potentials infectious diseases therapeutic values is of significant risen interest with biologically synthesized plant extracts derived silver nanoparticles on the greater focus. This research was aimed at determining the in vitro antimicrobial activity of silver nanoparticles synthesized from extracts of Terminalia avicennioides on antibiotic resistant Pseudomonas aeruginosa isolates from wounds. Standard phenotypic and genotypic techniques were used for the Isolation and identification of Pseudomonas aeruginosa isolates. Selected antibiotics, Terminalia avicennioides extracts and the extracts derived silver nanoparticles antimicrobial activities on the antibiotic resistant Pseudomonas aeruginosa were determined using standard tests methods. Findings showed the isolates to be resistant to 18.18% - 100% of the antibiotics used, but 100% sensitive to imipenem. Analysis of the plant extracts for bioactive compounds showed the presence of tannins, alkaloids, flavonoids, cardiac glycosides, phenols, saponins and terpenoids. Antimicrobial profile of Terminalia avicennioides extracts on the antibiotic resistant Pseudomonas aeruginosa isolates showed zones of growth inhibition ranged from 10.04±9.39 – 18.08±10.62 mm with no significant difference (P > 0.05), minimum inhibitory concentration ranged from 60.000+65.8281 -40.000 + 21.0821 mg/ml with no significant difference (p  0.05). The antimicrobial activity of the biologically synthesized silver nanoparticles on the antibiotic resistant Pseudomonas aeruginosa showed zone of growth inhibition ranged from 28.00 ± 13.51 – 53.00 ± 76.97 mm with no significant difference (p > 0.05). Terminalia avicennioides extracts and silver nanoparticles antimicrobial activity showed significant difference (p > 0.05). In comparison, the silver nanoparticles zones of growth inhibition was larger (28.39 ± 2.98 mm) than that of the extracts (16.83 ± 12.70 mm). This inferred that the synthesized silver nanoparticles possess potential of being used as a good chemotherapeutic agent for wound infections.},
 year = {2022}
}

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TY  - JOUR
T1  - In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates
AU  - Danjuma Lawal
AU  - Bobai Mathew
AU  - Sani Muhammad Nura
Y1  - 2022/08/17
PY  - 2022
N1  - https://doi.org/10.11648/j.jb.20220601.12
DO  - 10.11648/j.jb.20220601.12
T2  - Journal of Biomaterials
JF  - Journal of Biomaterials
JO  - Journal of Biomaterials
SP  - 5
EP  - 19
PB  - Science Publishing Group
SN  - 2640-2629
UR  - https://doi.org/10.11648/j.jb.20220601.12
AB  - Most available antimicrobials are now ineffective and the whole world healthcare system is currently under threat of antimicrobial resistant infections. Consequently, study of plant bioactive compounds with potentials infectious diseases therapeutic values is of significant risen interest with biologically synthesized plant extracts derived silver nanoparticles on the greater focus. This research was aimed at determining the in vitro antimicrobial activity of silver nanoparticles synthesized from extracts of Terminalia avicennioides on antibiotic resistant Pseudomonas aeruginosa isolates from wounds. Standard phenotypic and genotypic techniques were used for the Isolation and identification of Pseudomonas aeruginosa isolates. Selected antibiotics, Terminalia avicennioides extracts and the extracts derived silver nanoparticles antimicrobial activities on the antibiotic resistant Pseudomonas aeruginosa were determined using standard tests methods. Findings showed the isolates to be resistant to 18.18% - 100% of the antibiotics used, but 100% sensitive to imipenem. Analysis of the plant extracts for bioactive compounds showed the presence of tannins, alkaloids, flavonoids, cardiac glycosides, phenols, saponins and terpenoids. Antimicrobial profile of Terminalia avicennioides extracts on the antibiotic resistant Pseudomonas aeruginosa isolates showed zones of growth inhibition ranged from 10.04±9.39 – 18.08±10.62 mm with no significant difference (P > 0.05), minimum inhibitory concentration ranged from 60.000+65.8281 -40.000 + 21.0821 mg/ml with no significant difference (p  0.05). The antimicrobial activity of the biologically synthesized silver nanoparticles on the antibiotic resistant Pseudomonas aeruginosa showed zone of growth inhibition ranged from 28.00 ± 13.51 – 53.00 ± 76.97 mm with no significant difference (p > 0.05). Terminalia avicennioides extracts and silver nanoparticles antimicrobial activity showed significant difference (p > 0.05). In comparison, the silver nanoparticles zones of growth inhibition was larger (28.39 ± 2.98 mm) than that of the extracts (16.83 ± 12.70 mm). This inferred that the synthesized silver nanoparticles possess potential of being used as a good chemotherapeutic agent for wound infections.
VL  - 6
IS  - 1
ER  -

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