Involvement of ER Stress in Human Primary Pterygium

Sheng Zhou; Jing Yang

doi:doi:10.11648/j.ijovs.20190402.11

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Involvement of ER Stress in Human Primary Pterygium

Sheng Zhou, Jing Yang

Published in International Journal of Ophthalmology & Visual Science (Volume 4, Issue 2)

Received: 13 June 2019 Accepted: 2 July 2019 Published: 11 July 2019

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Abstract

Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78，spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.

Published in	International Journal of Ophthalmology & Visual Science (Volume 4, Issue 2)
DOI	10.11648/j.ijovs.20190402.11
Page(s)	30-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Primary Pterygium, Endoplasmic Reticulum Stress, Unfolded Protein Response, Inflammation, UPR Pathway Activation

References

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APA Style

Sheng Zhou, Jing Yang. (2019). Involvement of ER Stress in Human Primary Pterygium. International Journal of Ophthalmology & Visual Science, 4(2), 30-36. https://doi.org/10.11648/j.ijovs.20190402.11

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ACS Style

Sheng Zhou; Jing Yang. Involvement of ER Stress in Human Primary Pterygium. Int. J. Ophthalmol. Vis. Sci. 2019, 4(2), 30-36. doi: 10.11648/j.ijovs.20190402.11

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AMA Style

Sheng Zhou, Jing Yang. Involvement of ER Stress in Human Primary Pterygium. Int J Ophthalmol Vis Sci. 2019;4(2):30-36. doi: 10.11648/j.ijovs.20190402.11

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@article{10.11648/j.ijovs.20190402.11,
  author = {Sheng Zhou and Jing Yang},
  title = {Involvement of ER Stress in Human Primary Pterygium},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {4},
  number = {2},
  pages = {30-36},
  doi = {10.11648/j.ijovs.20190402.11},
  url = {https://doi.org/10.11648/j.ijovs.20190402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20190402.11},
  abstract = {Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78，spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.},
 year = {2019}
}

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TY  - JOUR
T1  - Involvement of ER Stress in Human Primary Pterygium
AU  - Sheng Zhou
AU  - Jing Yang
Y1  - 2019/07/11
PY  - 2019
N1  - https://doi.org/10.11648/j.ijovs.20190402.11
DO  - 10.11648/j.ijovs.20190402.11
T2  - International Journal of Ophthalmology & Visual Science
JF  - International Journal of Ophthalmology & Visual Science
JO  - International Journal of Ophthalmology & Visual Science
SP  - 30
EP  - 36
PB  - Science Publishing Group
SN  - 2637-3858
UR  - https://doi.org/10.11648/j.ijovs.20190402.11
AB  - Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78，spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.
VL  - 4
IS  - 2
ER  -

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Author Information

Sheng Zhou

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Jing Yang

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

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Plain Text BibTeX RIS

APA Style

Sheng Zhou, Jing Yang. (2019). Involvement of ER Stress in Human Primary Pterygium. International Journal of Ophthalmology & Visual Science, 4(2), 30-36. https://doi.org/10.11648/j.ijovs.20190402.11

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ACS Style

Sheng Zhou; Jing Yang. Involvement of ER Stress in Human Primary Pterygium. Int. J. Ophthalmol. Vis. Sci. 2019, 4(2), 30-36. doi: 10.11648/j.ijovs.20190402.11

Copy | Download

AMA Style

Sheng Zhou, Jing Yang. Involvement of ER Stress in Human Primary Pterygium. Int J Ophthalmol Vis Sci. 2019;4(2):30-36. doi: 10.11648/j.ijovs.20190402.11

Copy | Download

@article{10.11648/j.ijovs.20190402.11,
  author = {Sheng Zhou and Jing Yang},
  title = {Involvement of ER Stress in Human Primary Pterygium},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {4},
  number = {2},
  pages = {30-36},
  doi = {10.11648/j.ijovs.20190402.11},
  url = {https://doi.org/10.11648/j.ijovs.20190402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20190402.11},
  abstract = {Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78，spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.},
 year = {2019}
}

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TY  - JOUR
T1  - Involvement of ER Stress in Human Primary Pterygium
AU  - Sheng Zhou
AU  - Jing Yang
Y1  - 2019/07/11
PY  - 2019
N1  - https://doi.org/10.11648/j.ijovs.20190402.11
DO  - 10.11648/j.ijovs.20190402.11
T2  - International Journal of Ophthalmology & Visual Science
JF  - International Journal of Ophthalmology & Visual Science
JO  - International Journal of Ophthalmology & Visual Science
SP  - 30
EP  - 36
PB  - Science Publishing Group
SN  - 2637-3858
UR  - https://doi.org/10.11648/j.ijovs.20190402.11
AB  - Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78，spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.
VL  - 4
IS  - 2
ER  -

Copy | Download