In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.
Published in | International Journal of Ophthalmology & Visual Science (Volume 2, Issue 3) |
DOI | 10.11648/j.ijovs.20170203.12 |
Page(s) | 69-74 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2017. Published by Science Publishing Group |
Paracetamol (PC), Aceclofenac (AF), Serratio Peptidase (SP), RP-HPLC
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APA Style
Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. (2017). Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. International Journal of Ophthalmology & Visual Science, 2(3), 69-74. https://doi.org/10.11648/j.ijovs.20170203.12
ACS Style
Bhavani Podili; Mohan Seelam; Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int. J. Ophthalmol. Vis. Sci. 2017, 2(3), 69-74. doi: 10.11648/j.ijovs.20170203.12
@article{10.11648/j.ijovs.20170203.12, author = {Bhavani Podili and Mohan Seelam and Prasada Rao Kammela}, title = {Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC}, journal = {International Journal of Ophthalmology & Visual Science}, volume = {2}, number = {3}, pages = {69-74}, doi = {10.11648/j.ijovs.20170203.12}, url = {https://doi.org/10.11648/j.ijovs.20170203.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20170203.12}, abstract = {In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.}, year = {2017} }
TY - JOUR T1 - Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC AU - Bhavani Podili AU - Mohan Seelam AU - Prasada Rao Kammela Y1 - 2017/06/19 PY - 2017 N1 - https://doi.org/10.11648/j.ijovs.20170203.12 DO - 10.11648/j.ijovs.20170203.12 T2 - International Journal of Ophthalmology & Visual Science JF - International Journal of Ophthalmology & Visual Science JO - International Journal of Ophthalmology & Visual Science SP - 69 EP - 74 PB - Science Publishing Group SN - 2637-3858 UR - https://doi.org/10.11648/j.ijovs.20170203.12 AB - In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly. VL - 2 IS - 3 ER -