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Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC

Received: 30 March 2017     Accepted: 24 April 2017     Published: 19 June 2017
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Abstract

In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.

Published in International Journal of Ophthalmology & Visual Science (Volume 2, Issue 3)
DOI 10.11648/j.ijovs.20170203.12
Page(s) 69-74
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Paracetamol (PC), Aceclofenac (AF), Serratio Peptidase (SP), RP-HPLC

References
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[18] R. Yamazaki, S. Kawai, T. Matsuzaki, N. Kaneda, S. Hashimoto, T. Yokokuraet al. Aceclofenac blocks prostaglandin E2 production following its intracellular conversion into cyclooxygenase inhibitors. Eur. J Pharmacol. 1997, 329(2-3), 181-187.
[19] Brogden RN, Wiseman LR. Aceclofenac: a review of itspharmacodynamic properties and therapeutic potential inthe treatment of rheumatic disorders and in painmanagement. Drugs. 1996, 52(1), 113-124.
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  • APA Style

    Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. (2017). Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. International Journal of Ophthalmology & Visual Science, 2(3), 69-74. https://doi.org/10.11648/j.ijovs.20170203.12

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    ACS Style

    Bhavani Podili; Mohan Seelam; Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int. J. Ophthalmol. Vis. Sci. 2017, 2(3), 69-74. doi: 10.11648/j.ijovs.20170203.12

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    AMA Style

    Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int J Ophthalmol Vis Sci. 2017;2(3):69-74. doi: 10.11648/j.ijovs.20170203.12

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  • @article{10.11648/j.ijovs.20170203.12,
      author = {Bhavani Podili and Mohan Seelam and Prasada Rao Kammela},
      title = {Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC},
      journal = {International Journal of Ophthalmology & Visual Science},
      volume = {2},
      number = {3},
      pages = {69-74},
      doi = {10.11648/j.ijovs.20170203.12},
      url = {https://doi.org/10.11648/j.ijovs.20170203.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20170203.12},
      abstract = {In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC
    AU  - Bhavani Podili
    AU  - Mohan Seelam
    AU  - Prasada Rao Kammela
    Y1  - 2017/06/19
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijovs.20170203.12
    DO  - 10.11648/j.ijovs.20170203.12
    T2  - International Journal of Ophthalmology & Visual Science
    JF  - International Journal of Ophthalmology & Visual Science
    JO  - International Journal of Ophthalmology & Visual Science
    SP  - 69
    EP  - 74
    PB  - Science Publishing Group
    SN  - 2637-3858
    UR  - https://doi.org/10.11648/j.ijovs.20170203.12
    AB  - In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.
    VL  - 2
    IS  - 3
    ER  - 

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Author Information
  • Department of Chemistry, Acharya Nagarjuna University, Guntur, India

  • Department of Chemistry, Bapatla Engineering College, Bapatla, India

  • Department of Chemistry, Bapatla Engineering College, Bapatla, India

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