Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis

Li Peng; Xiao Xin; He Chuying; Song Jing; Long Jie

doi:doi:10.11648/j.ijdm.20230901.15

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Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie

Published in International Journal of Dental Medicine (Volume 9, Issue 1)

Received: 10 May 2023 Accepted: 30 May 2023 Published: 10 June 2023

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Abstract

Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.

Published in	International Journal of Dental Medicine (Volume 9, Issue 1)
DOI	10.11648/j.ijdm.20230901.15
Page(s)	23-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Distraction Ssteogenesis, Mandible, Micro-CT, Three-Dimensional Structure

References

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[16]	Karp NS, McCarthy JG, Schreiber JS, et al. Membranous bone lengthening: a serial histological study [J]. Ann Plast Surg, 1992, 29 (l): 2-7.
[17]	Komuro Y, Takato T, Harii K, et al. The histological analysis of distraction osteogenesis of the mandible in rabbits [J]. Plast Reconstr Surg, 1994, 94 (l): 152-159.
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APA Style

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. (2023). Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. International Journal of Dental Medicine, 9(1), 23-27. https://doi.org/10.11648/j.ijdm.20230901.15

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ACS Style

Li Peng; Xiao Xin; He Chuying; Song Jing; Long Jie. Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. Int. J. Dent. Med. 2023, 9(1), 23-27. doi: 10.11648/j.ijdm.20230901.15

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AMA Style

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. Int J Dent Med. 2023;9(1):23-27. doi: 10.11648/j.ijdm.20230901.15

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@article{10.11648/j.ijdm.20230901.15,
  author = {Li Peng and Xiao Xin and He Chuying and Song Jing and Long Jie},
  title = {Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis},
  journal = {International Journal of Dental Medicine},
  volume = {9},
  number = {1},
  pages = {23-27},
  doi = {10.11648/j.ijdm.20230901.15},
  url = {https://doi.org/10.11648/j.ijdm.20230901.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijdm.20230901.15},
  abstract = {Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.},
 year = {2023}
}

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TY  - JOUR
T1  - Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis
AU  - Li Peng
AU  - Xiao Xin
AU  - He Chuying
AU  - Song Jing
AU  - Long Jie
Y1  - 2023/06/10
PY  - 2023
N1  - https://doi.org/10.11648/j.ijdm.20230901.15
DO  - 10.11648/j.ijdm.20230901.15
T2  - International Journal of Dental Medicine
JF  - International Journal of Dental Medicine
JO  - International Journal of Dental Medicine
SP  - 23
EP  - 27
PB  - Science Publishing Group
SN  - 2472-1387
UR  - https://doi.org/10.11648/j.ijdm.20230901.15
AB  - Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.
VL  - 9
IS  - 1
ER  -

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Author Information

Li Peng

Department of VIP Clinic, Teaching Department, Affiliated Stomatology Hospital of Foshan University, Foshan Stomatology Hospital, Foshan, China
Xiao Xin

Department of VIP Clinic, Teaching Department, Affiliated Stomatology Hospital of Foshan University, Foshan Stomatology Hospital, Foshan, China
He Chuying

Department of VIP Clinic, Teaching Department, Affiliated Stomatology Hospital of Foshan University, Foshan Stomatology Hospital, Foshan, China
Song Jing

Department of VIP Clinic, Teaching Department, Affiliated Stomatology Hospital of Foshan University, Foshan Stomatology Hospital, Foshan, China
Long Jie

Department of Trauma and Plastic Surgery, West China School of Stomatology, Sichuan University, Chengdu, China

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APA Style

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. (2023). Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. International Journal of Dental Medicine, 9(1), 23-27. https://doi.org/10.11648/j.ijdm.20230901.15

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ACS Style

Li Peng; Xiao Xin; He Chuying; Song Jing; Long Jie. Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. Int. J. Dent. Med. 2023, 9(1), 23-27. doi: 10.11648/j.ijdm.20230901.15

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AMA Style

Li Peng, Xiao Xin, He Chuying, Song Jing, Long Jie. Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis. Int J Dent Med. 2023;9(1):23-27. doi: 10.11648/j.ijdm.20230901.15

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@article{10.11648/j.ijdm.20230901.15,
  author = {Li Peng and Xiao Xin and He Chuying and Song Jing and Long Jie},
  title = {Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis},
  journal = {International Journal of Dental Medicine},
  volume = {9},
  number = {1},
  pages = {23-27},
  doi = {10.11648/j.ijdm.20230901.15},
  url = {https://doi.org/10.11648/j.ijdm.20230901.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijdm.20230901.15},
  abstract = {Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Establishment and Micro-CT Histological Evaluation of the Animal Model for Rabbit Mandibular Distraction Osteogenesis
AU  - Li Peng
AU  - Xiao Xin
AU  - He Chuying
AU  - Song Jing
AU  - Long Jie
Y1  - 2023/06/10
PY  - 2023
N1  - https://doi.org/10.11648/j.ijdm.20230901.15
DO  - 10.11648/j.ijdm.20230901.15
T2  - International Journal of Dental Medicine
JF  - International Journal of Dental Medicine
JO  - International Journal of Dental Medicine
SP  - 23
EP  - 27
PB  - Science Publishing Group
SN  - 2472-1387
UR  - https://doi.org/10.11648/j.ijdm.20230901.15
AB  - Objective: To explore the application of self-made distractors to construct a unilateral DO model of rabbit mandible and evaluate the microstructure of bone specimens. Method: Designing a linear dual arm distractor and performing DO implantation surgery on 5 rabbits under local anesthesia. Stretching the mandible at rate of 1.0mm/day, 2 times/day for 10 days. After fixation for 3 months, taking mandibular specimens for gross observation, Micro-CT imaging and HE histological examination to analyze the bone density, maturity and three-dimensional structure of the new bone. Result: Except for one animal that died of unknown cause, the right mandibular body of the other animals was extended by about 10mm. The traction gap is completely filled with bright and red new bone with clear contours; Micro-CT reconstruction of three-dimensional bone images clearly shows high bone density in the gaps, with trabeculae arranging along the direction of distraction; HE staining shows that the bone trabeculae were connected into woven and layered bones, with a large number of concentric arranged bone plates surrounding the central canal to form the Haversian system, and osteoblasts proliferated actively. Conclusion: The use of Micro-CT and HE staining proves the success of the unilateral DO model of rabbit mandible using a self-made distractor, providing animal experiment for various biological factors and drug assisted methods to promote efficient DO in the later stage, and for its safe and effective clinical application.
VL  - 9
IS  - 1
ER  -

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