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Determination of Deproteinization Effect of 3N Contact Lenses Cleaner

Received: 8 August 2017     Accepted: 8 September 2017     Published: 16 October 2017
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Abstract

3N Contact Lenses Cleaner is a device utilized in effectively removing the protein deposition on contact lenses. It is independently developed by 3N Biological Technology Co., Ltd (3N Tech). Using the principle that proteins move in the electric field, 3N Contact Lenses Cleaner is able to adsorb proteins from the surface as well as the tiny holes of contact lenses, therefore, removes the protein deposition from the contact lenses effectively. In this study, we immersed contact lenses in artificial tears, and then proved 3N Contact Lenses Cleaner’s deproteinization effect with a qualitative detection using Coomassie Brilliant Blue G-250’s feature of indicating proteins. Meanwhile, we determined the device’s protein elution effect with Bradford method. The result indicates that 3N Contact Lenses Cleaner can distinctively remove the protein deposition from contact lenses.

Published in International Journal of Biomedical Materials Research (Volume 5, Issue 4)
DOI 10.11648/j.ijbmr.20170504.12
Page(s) 55-58
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

3N Contact Lenses Cleaner, Protein Movement, Deproteinization, Bradford Method

References
[1] Fowler SA, Korb DR, Allansmit Mr. Deposits on soft contact lenses of various water contents. CLAO, 1985, 11: 124-129.
[2] Compton SJ and Jones CG, Mechanism of dye response and interference in the Bradford protein assay, Anal Biochem, 1985, 151, 369–374.
[3] Bohnerr JL, Horbett TA, Ratner BD, et al. Adsorption of protein from artificial tear solution to contact lens materials. Invest Ophthalmol &Vis Sci, 1988, 29: 362- 373.
[4] Raymond I. Myers, et al. Quantity of protein deposited on hydrogel contact lenses and its relation to visible protein deposits. Optometry and Vision Science, 1991 68(10): 776-782.
[5] Richard NR, et al. Evaluation of tear deposits on contact lenses from patients with and without giant papillary conjunctivitis. CLAO J, 1992, 18: 143.
[6] Olafur G. et al. Identification of proteins in contact lens surface deposits by immunofluorescence microscopy. Arch Ophthalmol, 1985, 103: 196-197.
[7] Yan Wen, et al. A testing method of assessing the protein-removing effect of the contact lenses’ protein-removing care solution. Chinese Journal of Medical Instrumentation. 2006, 30(2).
[8] E. J. Castillo et al. Protein adsorption on hydrogels. Biomaterials, 1985, 6: 338-345.
[9] Liu Yi. Precipitates in contact lens lenses and related factors [J]. Foreign medicine: Division of Ophthalmology, 1998, 22 (6): 333-338.
[10] Bradford MM, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem, 72, 248–254 (1976).
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  • APA Style

    Bi-xia Sun, Yan-hong Wang. (2017). Determination of Deproteinization Effect of 3N Contact Lenses Cleaner. International Journal of Biomedical Materials Research, 5(4), 55-58. https://doi.org/10.11648/j.ijbmr.20170504.12

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    ACS Style

    Bi-xia Sun; Yan-hong Wang. Determination of Deproteinization Effect of 3N Contact Lenses Cleaner. Int. J. Biomed. Mater. Res. 2017, 5(4), 55-58. doi: 10.11648/j.ijbmr.20170504.12

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    AMA Style

    Bi-xia Sun, Yan-hong Wang. Determination of Deproteinization Effect of 3N Contact Lenses Cleaner. Int J Biomed Mater Res. 2017;5(4):55-58. doi: 10.11648/j.ijbmr.20170504.12

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  • @article{10.11648/j.ijbmr.20170504.12,
      author = {Bi-xia Sun and Yan-hong Wang},
      title = {Determination of Deproteinization Effect of 3N Contact Lenses Cleaner},
      journal = {International Journal of Biomedical Materials Research},
      volume = {5},
      number = {4},
      pages = {55-58},
      doi = {10.11648/j.ijbmr.20170504.12},
      url = {https://doi.org/10.11648/j.ijbmr.20170504.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20170504.12},
      abstract = {3N Contact Lenses Cleaner is a device utilized in effectively removing the protein deposition on contact lenses. It is independently developed by 3N Biological Technology Co., Ltd (3N Tech). Using the principle that proteins move in the electric field, 3N Contact Lenses Cleaner is able to adsorb proteins from the surface as well as the tiny holes of contact lenses, therefore, removes the protein deposition from the contact lenses effectively. In this study, we immersed contact lenses in artificial tears, and then proved 3N Contact Lenses Cleaner’s deproteinization effect with a qualitative detection using Coomassie Brilliant Blue G-250’s feature of indicating proteins. Meanwhile, we determined the device’s protein elution effect with Bradford method. The result indicates that 3N Contact Lenses Cleaner can distinctively remove the protein deposition from contact lenses.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Determination of Deproteinization Effect of 3N Contact Lenses Cleaner
    AU  - Bi-xia Sun
    AU  - Yan-hong Wang
    Y1  - 2017/10/16
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    DO  - 10.11648/j.ijbmr.20170504.12
    T2  - International Journal of Biomedical Materials Research
    JF  - International Journal of Biomedical Materials Research
    JO  - International Journal of Biomedical Materials Research
    SP  - 55
    EP  - 58
    PB  - Science Publishing Group
    SN  - 2330-7579
    UR  - https://doi.org/10.11648/j.ijbmr.20170504.12
    AB  - 3N Contact Lenses Cleaner is a device utilized in effectively removing the protein deposition on contact lenses. It is independently developed by 3N Biological Technology Co., Ltd (3N Tech). Using the principle that proteins move in the electric field, 3N Contact Lenses Cleaner is able to adsorb proteins from the surface as well as the tiny holes of contact lenses, therefore, removes the protein deposition from the contact lenses effectively. In this study, we immersed contact lenses in artificial tears, and then proved 3N Contact Lenses Cleaner’s deproteinization effect with a qualitative detection using Coomassie Brilliant Blue G-250’s feature of indicating proteins. Meanwhile, we determined the device’s protein elution effect with Bradford method. The result indicates that 3N Contact Lenses Cleaner can distinctively remove the protein deposition from contact lenses.
    VL  - 5
    IS  - 4
    ER  - 

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Author Information
  • Suzhou 3N Biological Technology Co., Ltd (3N Tech), Suzhou, China

  • Institute of Zoology, Chinese Academy of Sciences, Beijing, China

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