Detection of Salt Tolerant Gene (<i>TaNIP</i>) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions

Ibrahim Ismail Al-Mashhadani; Duha Mysire Majeed; Eman Noaman Ismail; Maysaa Sameer Kadhim

doi:doi:10.11648/j.ijaas.20160201.12

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Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions

Ibrahim Ismail Al-Mashhadani, Duha Mysire Majeed, Eman Noaman Ismail, Maysaa Sameer Kadhim

Published in International Journal of Applied Agricultural Sciences (Volume 2, Issue 1)

Received: 29 December 2015 Accepted: 4 January 2016 Published: 23 January 2016

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Abstract

High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.

Published in	International Journal of Applied Agricultural Sciences (Volume 2, Issue 1)
DOI	10.11648/j.ijaas.20160201.12
Page(s)	12-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Wheat Genotype, Salt Tolerance, TaNIP Gene, Real-Time PCR

References

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APA Style

Ibrahim Ismail Al-Mashhadani, Duha Mysire Majeed, Eman Noaman Ismail, Maysaa Sameer Kadhim. (2016). Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions. International Journal of Applied Agricultural Sciences, 2(1), 12-16. https://doi.org/10.11648/j.ijaas.20160201.12

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ACS Style

Ibrahim Ismail Al-Mashhadani; Duha Mysire Majeed; Eman Noaman Ismail; Maysaa Sameer Kadhim. Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions. Int. J. Appl. Agric. Sci. 2016, 2(1), 12-16. doi: 10.11648/j.ijaas.20160201.12

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AMA Style

Ibrahim Ismail Al-Mashhadani, Duha Mysire Majeed, Eman Noaman Ismail, Maysaa Sameer Kadhim. Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions. Int J Appl Agric Sci. 2016;2(1):12-16. doi: 10.11648/j.ijaas.20160201.12

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@article{10.11648/j.ijaas.20160201.12,
  author = {Ibrahim Ismail Al-Mashhadani and Duha Mysire Majeed and Eman Noaman Ismail and Maysaa Sameer Kadhim},
  title = {Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {2},
  number = {1},
  pages = {12-16},
  doi = {10.11648/j.ijaas.20160201.12},
  url = {https://doi.org/10.11648/j.ijaas.20160201.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20160201.12},
  abstract = {High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.},
 year = {2016}
}

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TY  - JOUR
T1  - Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions
AU  - Ibrahim Ismail Al-Mashhadani
AU  - Duha Mysire Majeed
AU  - Eman Noaman Ismail
AU  - Maysaa Sameer Kadhim
Y1  - 2016/01/23
PY  - 2016
N1  - https://doi.org/10.11648/j.ijaas.20160201.12
DO  - 10.11648/j.ijaas.20160201.12
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 12
EP  - 16
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20160201.12
AB  - High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.
VL  - 2
IS  - 1
ER  -

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Author Information

Ibrahim Ismail Al-Mashhadani

Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
Duha Mysire Majeed

Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
Eman Noaman Ismail

Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
Maysaa Sameer Kadhim

Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq

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Plain Text BibTeX RIS

APA Style

Ibrahim Ismail Al-Mashhadani, Duha Mysire Majeed, Eman Noaman Ismail, Maysaa Sameer Kadhim. (2016). Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions. International Journal of Applied Agricultural Sciences, 2(1), 12-16. https://doi.org/10.11648/j.ijaas.20160201.12

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ACS Style

Ibrahim Ismail Al-Mashhadani; Duha Mysire Majeed; Eman Noaman Ismail; Maysaa Sameer Kadhim. Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions. Int. J. Appl. Agric. Sci. 2016, 2(1), 12-16. doi: 10.11648/j.ijaas.20160201.12

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AMA Style

Ibrahim Ismail Al-Mashhadani, Duha Mysire Majeed, Eman Noaman Ismail, Maysaa Sameer Kadhim. Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions. Int J Appl Agric Sci. 2016;2(1):12-16. doi: 10.11648/j.ijaas.20160201.12

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@article{10.11648/j.ijaas.20160201.12,
  author = {Ibrahim Ismail Al-Mashhadani and Duha Mysire Majeed and Eman Noaman Ismail and Maysaa Sameer Kadhim},
  title = {Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {2},
  number = {1},
  pages = {12-16},
  doi = {10.11648/j.ijaas.20160201.12},
  url = {https://doi.org/10.11648/j.ijaas.20160201.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20160201.12},
  abstract = {High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.},
 year = {2016}
}

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TY  - JOUR
T1  - Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions
AU  - Ibrahim Ismail Al-Mashhadani
AU  - Duha Mysire Majeed
AU  - Eman Noaman Ismail
AU  - Maysaa Sameer Kadhim
Y1  - 2016/01/23
PY  - 2016
N1  - https://doi.org/10.11648/j.ijaas.20160201.12
DO  - 10.11648/j.ijaas.20160201.12
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 12
EP  - 16
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20160201.12
AB  - High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.
VL  - 2
IS  - 1
ER  -

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