Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis

Fu Xianzhao; Li Chunyan; Huang Wenhua; Li Xingchan; Qiu Haixian; Huang Guangming; Cao Qiuxia; Lu Mian

doi:doi:10.11648/j.ajcem.20190705.12

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Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis

Fu Xianzhao, Li Chunyan, Huang Wenhua, Li Xingchan, Qiu Haixian, Huang Guangming, Cao Qiuxia, Lu Mian

Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 5)

Received: 19 September 2019 Accepted: 28 September 2019 Published: 15 October 2019

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Abstract

To explore the mechanism of Huoxue Jiedu Jiangtang Recipe (HJJR) in alleviating endoplasmic reticulum stress (ERS) - inflammation, inhibiting ERS apoptotic bypass of CHOP and alleviating diabetic atherosclerosis (AS). Diabetic AS rats were duplicated by feeding of high lipid diet and intraperitoneal injection of streptozotocin. After diabetes modeled successfully, the rats were randomly divided into model group, low-dose HJJR group (HJJR₁), high-dose HJJR group (HJJR₂) and western medicine group (Gliquidone+ Benazepril), and accepted corresponding drugs for 2 months respectively. The same batch of rats were taken as normal control group and fed with common diet. The levels of glycosylated hemoglobin (GHb), blood lipid, fasting serum insulin (FINS) and insulin resistance index (IRI) were measured in each group. The contents of inflammatory factors TNF-α and IL-6 were detected by immunohistochemistry. The mRNA transcription of GRP78 and caspase-12 were tested by reverse- transcription polymerase chain reaction (RT-PCR). The apoptotic level of aortic cells was checked by TUNEL. Compared with the model group, all drug groups could significantly reduce GHb, IRI, TG and LDL-C (P<0.05), increase FINS and HDL-C (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), reduce inflammatory factors TNF-a, IL-6, and decreased aortic apoptosis index (AI) (P<0.05). Compared with the western medicine group, the effect of HJJR2 group was more significant (P<0.05). HJJR could alleviate insulin resistance, correct lipid metabolism disorder, depress endoplasmic reticulum stress-induced inflammatory reaction, and inhibit endoplasmic reticulum stress-induced apoptotic bypass of CHOP in arterial cells. The therapeutic effect is dose dependent.

Published in	American Journal of Clinical and Experimental Medicine (Volume 7, Issue 5)
DOI	10.11648/j.ajcem.20190705.12
Page(s)	111-118
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Diabetes, Atherosclerosis, Endoplasmic Reticulum Stress, CCAAT Enhance-Binding Protein Homologous Protein, Huoxue Jiedu Jiangtang Recipe

References

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[3]	Fu Xianzhao, Huang Zhenfeng, Huang Wenhua, et al. Review and Prospect of the Preventive Effect of “Supplementing Qi and Nourishing Yin, Activating Blood Circulation and Detoxifying” on Diabetes Mellitus Complicated with Acute Coronary Syndrome. American Journal of Clinical and Experimental Medicine. 2018, 6 (4): 87-93.
[4]	Fu Xianzhao, Huang Wenhua, Li Chunyan, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. American Journal of Clinical and Experimental Medicine. 2018, 6 (5): 118-124.
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APA Style

Fu Xianzhao, Li Chunyan, Huang Wenhua, Li Xingchan, Qiu Haixian, et al. (2019). Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis. American Journal of Clinical and Experimental Medicine, 7(5), 111-118. https://doi.org/10.11648/j.ajcem.20190705.12

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ACS Style

Fu Xianzhao; Li Chunyan; Huang Wenhua; Li Xingchan; Qiu Haixian, et al. Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis. Am. J. Clin. Exp. Med. 2019, 7(5), 111-118. doi: 10.11648/j.ajcem.20190705.12

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AMA Style

Fu Xianzhao, Li Chunyan, Huang Wenhua, Li Xingchan, Qiu Haixian, et al. Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis. Am J Clin Exp Med. 2019;7(5):111-118. doi: 10.11648/j.ajcem.20190705.12

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@article{10.11648/j.ajcem.20190705.12,
  author = {Fu Xianzhao and Li Chunyan and Huang Wenhua and Li Xingchan and Qiu Haixian and Huang Guangming and Cao Qiuxia and Lu Mian},
  title = {Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {5},
  pages = {111-118},
  doi = {10.11648/j.ajcem.20190705.12},
  url = {https://doi.org/10.11648/j.ajcem.20190705.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190705.12},
  abstract = {To explore the mechanism of Huoxue Jiedu Jiangtang Recipe (HJJR) in alleviating endoplasmic reticulum stress (ERS) - inflammation, inhibiting ERS apoptotic bypass of CHOP and alleviating diabetic atherosclerosis (AS). Diabetic AS rats were duplicated by feeding of high lipid diet and intraperitoneal injection of streptozotocin. After diabetes modeled successfully, the rats were randomly divided into model group, low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2) and western medicine group (Gliquidone+ Benazepril), and accepted corresponding drugs for 2 months respectively. The same batch of rats were taken as normal control group and fed with common diet. The levels of glycosylated hemoglobin (GHb), blood lipid, fasting serum insulin (FINS) and insulin resistance index (IRI) were measured in each group. The contents of inflammatory factors TNF-α and IL-6 were detected by immunohistochemistry. The mRNA transcription of GRP78 and caspase-12 were tested by reverse- transcription polymerase chain reaction (RT-PCR). The apoptotic level of aortic cells was checked by TUNEL. Compared with the model group, all drug groups could significantly reduce GHb, IRI, TG and LDL-C (P<0.05), increase FINS and HDL-C (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), reduce inflammatory factors TNF-a, IL-6, and decreased aortic apoptosis index (AI) (P<0.05). Compared with the western medicine group, the effect of HJJR2 group was more significant (P<0.05). HJJR could alleviate insulin resistance, correct lipid metabolism disorder, depress endoplasmic reticulum stress-induced inflammatory reaction, and inhibit endoplasmic reticulum stress-induced apoptotic bypass of CHOP in arterial cells. The therapeutic effect is dose dependent.},
 year = {2019}
}

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TY  - JOUR
T1  - Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis
AU  - Fu Xianzhao
AU  - Li Chunyan
AU  - Huang Wenhua
AU  - Li Xingchan
AU  - Qiu Haixian
AU  - Huang Guangming
AU  - Cao Qiuxia
AU  - Lu Mian
Y1  - 2019/10/15
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190705.12
DO  - 10.11648/j.ajcem.20190705.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 111
EP  - 118
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190705.12
AB  - To explore the mechanism of Huoxue Jiedu Jiangtang Recipe (HJJR) in alleviating endoplasmic reticulum stress (ERS) - inflammation, inhibiting ERS apoptotic bypass of CHOP and alleviating diabetic atherosclerosis (AS). Diabetic AS rats were duplicated by feeding of high lipid diet and intraperitoneal injection of streptozotocin. After diabetes modeled successfully, the rats were randomly divided into model group, low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2) and western medicine group (Gliquidone+ Benazepril), and accepted corresponding drugs for 2 months respectively. The same batch of rats were taken as normal control group and fed with common diet. The levels of glycosylated hemoglobin (GHb), blood lipid, fasting serum insulin (FINS) and insulin resistance index (IRI) were measured in each group. The contents of inflammatory factors TNF-α and IL-6 were detected by immunohistochemistry. The mRNA transcription of GRP78 and caspase-12 were tested by reverse- transcription polymerase chain reaction (RT-PCR). The apoptotic level of aortic cells was checked by TUNEL. Compared with the model group, all drug groups could significantly reduce GHb, IRI, TG and LDL-C (P<0.05), increase FINS and HDL-C (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), reduce inflammatory factors TNF-a, IL-6, and decreased aortic apoptosis index (AI) (P<0.05). Compared with the western medicine group, the effect of HJJR2 group was more significant (P<0.05). HJJR could alleviate insulin resistance, correct lipid metabolism disorder, depress endoplasmic reticulum stress-induced inflammatory reaction, and inhibit endoplasmic reticulum stress-induced apoptotic bypass of CHOP in arterial cells. The therapeutic effect is dose dependent.
VL  - 7
IS  - 5
ER  -

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Author Information

Fu Xianzhao

Clinic Medical College, Youjiang Medical National College, Baise, China
Li Chunyan

Clinic Medical College, Youjiang Medical National College, Baise, China
Huang Wenhua

Clinic Medical College, Youjiang Medical National College, Baise, China
Li Xingchan

Clinic Medical College, Youjiang Medical National College, Baise, China
Qiu Haixian

Clinic Medical College, Youjiang Medical National College, Baise, China
Huang Guangming

Clinic Medical College, Youjiang Medical National College, Baise, China
Cao Qiuxia

Clinic Medical College, Youjiang Medical National College, Baise, China
Lu Mian

Clinic Medical College, Youjiang Medical National College, Baise, China

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Plain Text BibTeX RIS

APA Style

Fu Xianzhao, Li Chunyan, Huang Wenhua, Li Xingchan, Qiu Haixian, et al. (2019). Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis. American Journal of Clinical and Experimental Medicine, 7(5), 111-118. https://doi.org/10.11648/j.ajcem.20190705.12

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ACS Style

Fu Xianzhao; Li Chunyan; Huang Wenhua; Li Xingchan; Qiu Haixian, et al. Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis. Am. J. Clin. Exp. Med. 2019, 7(5), 111-118. doi: 10.11648/j.ajcem.20190705.12

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AMA Style

Fu Xianzhao, Li Chunyan, Huang Wenhua, Li Xingchan, Qiu Haixian, et al. Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis. Am J Clin Exp Med. 2019;7(5):111-118. doi: 10.11648/j.ajcem.20190705.12

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@article{10.11648/j.ajcem.20190705.12,
  author = {Fu Xianzhao and Li Chunyan and Huang Wenhua and Li Xingchan and Qiu Haixian and Huang Guangming and Cao Qiuxia and Lu Mian},
  title = {Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {5},
  pages = {111-118},
  doi = {10.11648/j.ajcem.20190705.12},
  url = {https://doi.org/10.11648/j.ajcem.20190705.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190705.12},
  abstract = {To explore the mechanism of Huoxue Jiedu Jiangtang Recipe (HJJR) in alleviating endoplasmic reticulum stress (ERS) - inflammation, inhibiting ERS apoptotic bypass of CHOP and alleviating diabetic atherosclerosis (AS). Diabetic AS rats were duplicated by feeding of high lipid diet and intraperitoneal injection of streptozotocin. After diabetes modeled successfully, the rats were randomly divided into model group, low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2) and western medicine group (Gliquidone+ Benazepril), and accepted corresponding drugs for 2 months respectively. The same batch of rats were taken as normal control group and fed with common diet. The levels of glycosylated hemoglobin (GHb), blood lipid, fasting serum insulin (FINS) and insulin resistance index (IRI) were measured in each group. The contents of inflammatory factors TNF-α and IL-6 were detected by immunohistochemistry. The mRNA transcription of GRP78 and caspase-12 were tested by reverse- transcription polymerase chain reaction (RT-PCR). The apoptotic level of aortic cells was checked by TUNEL. Compared with the model group, all drug groups could significantly reduce GHb, IRI, TG and LDL-C (P<0.05), increase FINS and HDL-C (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), reduce inflammatory factors TNF-a, IL-6, and decreased aortic apoptosis index (AI) (P<0.05). Compared with the western medicine group, the effect of HJJR2 group was more significant (P<0.05). HJJR could alleviate insulin resistance, correct lipid metabolism disorder, depress endoplasmic reticulum stress-induced inflammatory reaction, and inhibit endoplasmic reticulum stress-induced apoptotic bypass of CHOP in arterial cells. The therapeutic effect is dose dependent.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis
AU  - Fu Xianzhao
AU  - Li Chunyan
AU  - Huang Wenhua
AU  - Li Xingchan
AU  - Qiu Haixian
AU  - Huang Guangming
AU  - Cao Qiuxia
AU  - Lu Mian
Y1  - 2019/10/15
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190705.12
DO  - 10.11648/j.ajcem.20190705.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 111
EP  - 118
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190705.12
AB  - To explore the mechanism of Huoxue Jiedu Jiangtang Recipe (HJJR) in alleviating endoplasmic reticulum stress (ERS) - inflammation, inhibiting ERS apoptotic bypass of CHOP and alleviating diabetic atherosclerosis (AS). Diabetic AS rats were duplicated by feeding of high lipid diet and intraperitoneal injection of streptozotocin. After diabetes modeled successfully, the rats were randomly divided into model group, low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2) and western medicine group (Gliquidone+ Benazepril), and accepted corresponding drugs for 2 months respectively. The same batch of rats were taken as normal control group and fed with common diet. The levels of glycosylated hemoglobin (GHb), blood lipid, fasting serum insulin (FINS) and insulin resistance index (IRI) were measured in each group. The contents of inflammatory factors TNF-α and IL-6 were detected by immunohistochemistry. The mRNA transcription of GRP78 and caspase-12 were tested by reverse- transcription polymerase chain reaction (RT-PCR). The apoptotic level of aortic cells was checked by TUNEL. Compared with the model group, all drug groups could significantly reduce GHb, IRI, TG and LDL-C (P<0.05), increase FINS and HDL-C (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), reduce inflammatory factors TNF-a, IL-6, and decreased aortic apoptosis index (AI) (P<0.05). Compared with the western medicine group, the effect of HJJR2 group was more significant (P<0.05). HJJR could alleviate insulin resistance, correct lipid metabolism disorder, depress endoplasmic reticulum stress-induced inflammatory reaction, and inhibit endoplasmic reticulum stress-induced apoptotic bypass of CHOP in arterial cells. The therapeutic effect is dose dependent.
VL  - 7
IS  - 5
ER  -

Copy | Download