Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure

Junia Kirana; Khairuddin Djawad; Siswanto Wahab

doi:doi:10.11648/j.ajcem.20170504.11

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Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure

Junia Kirana, Khairuddin Djawad, Siswanto Wahab

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 4)

Received: 28 February 2017 Accepted: 24 March 2017 Published: 2 June 2017

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Abstract

Skin cancer incidence is directly proportional to malignancies in other organs and represents a health problem. This study aims to evaluate the protective effect of topical cocoa extract application on PCNA expression in mice receiving DMBA exposure. This study was conducted in Animal Laboratory for DMBA administration and cocoa extract application, Bimolecular Laboratory of Hasanuddin University as the location of ELISA evaluation. The study used animal experimental method with pure experimental design to find out the role of topical cocoa seeds extract on the skin of mice receiving DMBA application in one week. Twenty mice were divided into 4 treatment groups: first group was control without skin protector, second group with ethanol application, third group with topical cocoa extract application 400 ppm, and fourth group was treated with 800 ppm topical cocoa with 100 ug DMBA exposure three times per week. After one week the treatment was terminated and skin biopsy was excised for PCNA expression evaluation using ELISA. Study findings indicate that 400 ppm and 800 ppm topical cocoa extract had a protective effect on PCNA expression with 400 ppm as the most effective dose.

Published in	American Journal of Clinical and Experimental Medicine (Volume 5, Issue 4)
DOI	10.11648/j.ajcem.20170504.11
Page(s)	102-107
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

DMBA, Cocoa Extract, PCNA

References

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APA Style

Junia Kirana, Khairuddin Djawad, Siswanto Wahab. (2017). Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure. American Journal of Clinical and Experimental Medicine, 5(4), 102-107. https://doi.org/10.11648/j.ajcem.20170504.11

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ACS Style

Junia Kirana; Khairuddin Djawad; Siswanto Wahab. Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure. Am. J. Clin. Exp. Med. 2017, 5(4), 102-107. doi: 10.11648/j.ajcem.20170504.11

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AMA Style

Junia Kirana, Khairuddin Djawad, Siswanto Wahab. Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure. Am J Clin Exp Med. 2017;5(4):102-107. doi: 10.11648/j.ajcem.20170504.11

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@article{10.11648/j.ajcem.20170504.11,
  author = {Junia Kirana and Khairuddin Djawad and Siswanto Wahab},
  title = {Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {4},
  pages = {102-107},
  doi = {10.11648/j.ajcem.20170504.11},
  url = {https://doi.org/10.11648/j.ajcem.20170504.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170504.11},
  abstract = {Skin cancer incidence is directly proportional to malignancies in other organs and represents a health problem. This study aims to evaluate the protective effect of topical cocoa extract application on PCNA expression in mice receiving DMBA exposure. This study was conducted in Animal Laboratory for DMBA administration and cocoa extract application, Bimolecular Laboratory of Hasanuddin University as the location of ELISA evaluation. The study used animal experimental method with pure experimental design to find out the role of topical cocoa seeds extract on the skin of mice receiving DMBA application in one week. Twenty mice were divided into 4 treatment groups: first group was control without skin protector, second group with ethanol application, third group with topical cocoa extract application 400 ppm, and fourth group was treated with 800 ppm topical cocoa with 100 ug DMBA exposure three times per week. After one week the treatment was terminated and skin biopsy was excised for PCNA expression evaluation using ELISA. Study findings indicate that 400 ppm and 800 ppm topical cocoa extract had a protective effect on PCNA expression with 400 ppm as the most effective dose.},
 year = {2017}
}

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TY  - JOUR
T1  - Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure
AU  - Junia Kirana
AU  - Khairuddin Djawad
AU  - Siswanto Wahab
Y1  - 2017/06/02
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170504.11
DO  - 10.11648/j.ajcem.20170504.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 102
EP  - 107
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170504.11
AB  - Skin cancer incidence is directly proportional to malignancies in other organs and represents a health problem. This study aims to evaluate the protective effect of topical cocoa extract application on PCNA expression in mice receiving DMBA exposure. This study was conducted in Animal Laboratory for DMBA administration and cocoa extract application, Bimolecular Laboratory of Hasanuddin University as the location of ELISA evaluation. The study used animal experimental method with pure experimental design to find out the role of topical cocoa seeds extract on the skin of mice receiving DMBA application in one week. Twenty mice were divided into 4 treatment groups: first group was control without skin protector, second group with ethanol application, third group with topical cocoa extract application 400 ppm, and fourth group was treated with 800 ppm topical cocoa with 100 ug DMBA exposure three times per week. After one week the treatment was terminated and skin biopsy was excised for PCNA expression evaluation using ELISA. Study findings indicate that 400 ppm and 800 ppm topical cocoa extract had a protective effect on PCNA expression with 400 ppm as the most effective dose.
VL  - 5
IS  - 4
ER  -

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Author Information

Junia Kirana

Department of Dermatology and Venereology, Hasanuddin University, Makassar, Indonesia
Khairuddin Djawad

Department of Dermatology and Venereology, Hasanuddin University, Makassar, Indonesia
Siswanto Wahab

Department of Dermatology and Venereology, Hasanuddin University, Makassar, Indonesia

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Plain Text BibTeX RIS

APA Style

Junia Kirana, Khairuddin Djawad, Siswanto Wahab. (2017). Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure. American Journal of Clinical and Experimental Medicine, 5(4), 102-107. https://doi.org/10.11648/j.ajcem.20170504.11

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ACS Style

Junia Kirana; Khairuddin Djawad; Siswanto Wahab. Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure. Am. J. Clin. Exp. Med. 2017, 5(4), 102-107. doi: 10.11648/j.ajcem.20170504.11

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AMA Style

Junia Kirana, Khairuddin Djawad, Siswanto Wahab. Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure. Am J Clin Exp Med. 2017;5(4):102-107. doi: 10.11648/j.ajcem.20170504.11

Copy | Download

@article{10.11648/j.ajcem.20170504.11,
  author = {Junia Kirana and Khairuddin Djawad and Siswanto Wahab},
  title = {Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {4},
  pages = {102-107},
  doi = {10.11648/j.ajcem.20170504.11},
  url = {https://doi.org/10.11648/j.ajcem.20170504.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170504.11},
  abstract = {Skin cancer incidence is directly proportional to malignancies in other organs and represents a health problem. This study aims to evaluate the protective effect of topical cocoa extract application on PCNA expression in mice receiving DMBA exposure. This study was conducted in Animal Laboratory for DMBA administration and cocoa extract application, Bimolecular Laboratory of Hasanuddin University as the location of ELISA evaluation. The study used animal experimental method with pure experimental design to find out the role of topical cocoa seeds extract on the skin of mice receiving DMBA application in one week. Twenty mice were divided into 4 treatment groups: first group was control without skin protector, second group with ethanol application, third group with topical cocoa extract application 400 ppm, and fourth group was treated with 800 ppm topical cocoa with 100 ug DMBA exposure three times per week. After one week the treatment was terminated and skin biopsy was excised for PCNA expression evaluation using ELISA. Study findings indicate that 400 ppm and 800 ppm topical cocoa extract had a protective effect on PCNA expression with 400 ppm as the most effective dose.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Protective Effect of Cocoa Seeds Extract Application on PCNA Expression in Albino Mice Receiving DMBA Exposure
AU  - Junia Kirana
AU  - Khairuddin Djawad
AU  - Siswanto Wahab
Y1  - 2017/06/02
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170504.11
DO  - 10.11648/j.ajcem.20170504.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 102
EP  - 107
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170504.11
AB  - Skin cancer incidence is directly proportional to malignancies in other organs and represents a health problem. This study aims to evaluate the protective effect of topical cocoa extract application on PCNA expression in mice receiving DMBA exposure. This study was conducted in Animal Laboratory for DMBA administration and cocoa extract application, Bimolecular Laboratory of Hasanuddin University as the location of ELISA evaluation. The study used animal experimental method with pure experimental design to find out the role of topical cocoa seeds extract on the skin of mice receiving DMBA application in one week. Twenty mice were divided into 4 treatment groups: first group was control without skin protector, second group with ethanol application, third group with topical cocoa extract application 400 ppm, and fourth group was treated with 800 ppm topical cocoa with 100 ug DMBA exposure three times per week. After one week the treatment was terminated and skin biopsy was excised for PCNA expression evaluation using ELISA. Study findings indicate that 400 ppm and 800 ppm topical cocoa extract had a protective effect on PCNA expression with 400 ppm as the most effective dose.
VL  - 5
IS  - 4
ER  -

Copy | Download