The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke

Chitanava Tamara; Egiev Ivan; Chechelian Valerii; Dzhopua Maksim; Trofimenko Artem; Zanin Sergey; Turovaya Alla; Kade Azamat

doi:doi:10.11648/j.ajcem.20170503.15

The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke

Chitanava Tamara, Egiev Ivan, Chechelian Valerii, Dzhopua Maksim, Trofimenko Artem, Zanin Sergey, Turovaya Alla, Kade Azamat

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 3)

Received: 27 February 2017 Accepted: 5 April 2017 Published: 9 May 2017

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Abstract

The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).

Published in	American Journal of Clinical and Experimental Medicine (Volume 5, Issue 3)
DOI	10.11648/j.ajcem.20170503.15
Page(s)	93-96
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

D-Asparagine, Glial Cicatrix, Spinal Cord Injury, Photothrombosis, Micropreparations, Rats

References

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[2]	Bryukhovetskiy I. S., Dyuizen I. V. Morfochemical charachteristic of the spinal cord of rats after the transplantation of polymer collagen neuromatrix “Sferogel – E” ^TM with the incorporated parietal neuroepithelial cells // Genes and cells. – 2008. – №2. – 57 – 62 pages.
[3]	Morozov I. N., Mlyavikx S. G. Epidemiology of spinal cord injury (review) // Medical Almanac. – 2011. – №4. – 157-159 pages.
[4]	Trofimenko A. I.  - endorphin and cytokine profile in dynamics of experimental ischemic stroke // Modern problems of science and education – 2014. – №6. – 1125 page.
[5]	Fadeeva V. E. Investigation the safety of usage D-asparagine in experimental model of spinal stroke in rats // In: Medical science and Public health. – 2016. – 73 – 74 pages.
[6]	Chitanava T. V. The influence of D-asparagine on the formation of glial cicatrix in experimental spinal stroke in rats // In: Medical science and Public health. – 2016. – 37 – 39 pages.
[7]	Kennedy H. S. A method for removing the brain and spinal cord as one unit from adult mice and rats // Lab Anim (NY). – 2011. – Feb; 40(2):53-7. doi: 10.1038/laban 0211-53.
[8]	Kierman J. A. Histological and histochemical methods. Theory and practice. – London: Scion Publishing Ltd. – 2008. – 606 page.
[9]	Von Euler M. Morphological characterization of the evolving rat spinal cord injury after photochemically induced ischemia // Acta Neuropathol. – 1997 Sep; 94(3). – 232-239 pages.
[10]	Wolosker H, D'Aniello A, Snyder SH. D-aspartate disposition in neuronal and endocrine tissues: ontogeny, biosynthesis and release // Neuroscience. – 2000. – 100(1). – 183-189 pages.

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APA Style

Chitanava Tamara, Egiev Ivan, Chechelian Valerii, Dzhopua Maksim, Trofimenko Artem, et al. (2017). The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. American Journal of Clinical and Experimental Medicine, 5(3), 93-96. https://doi.org/10.11648/j.ajcem.20170503.15

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ACS Style

Chitanava Tamara; Egiev Ivan; Chechelian Valerii; Dzhopua Maksim; Trofimenko Artem, et al. The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. Am. J. Clin. Exp. Med. 2017, 5(3), 93-96. doi: 10.11648/j.ajcem.20170503.15

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AMA Style

Chitanava Tamara, Egiev Ivan, Chechelian Valerii, Dzhopua Maksim, Trofimenko Artem, et al. The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. Am J Clin Exp Med. 2017;5(3):93-96. doi: 10.11648/j.ajcem.20170503.15

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@article{10.11648/j.ajcem.20170503.15,
  author = {Chitanava Tamara and Egiev Ivan and Chechelian Valerii and Dzhopua Maksim and Trofimenko Artem and Zanin Sergey and Turovaya Alla and Kade Azamat},
  title = {The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {3},
  pages = {93-96},
  doi = {10.11648/j.ajcem.20170503.15},
  url = {https://doi.org/10.11648/j.ajcem.20170503.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170503.15},
  abstract = {The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).},
 year = {2017}
}

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TY  - JOUR
T1  - The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke
AU  - Chitanava Tamara
AU  - Egiev Ivan
AU  - Chechelian Valerii
AU  - Dzhopua Maksim
AU  - Trofimenko Artem
AU  - Zanin Sergey
AU  - Turovaya Alla
AU  - Kade Azamat
Y1  - 2017/05/09
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170503.15
DO  - 10.11648/j.ajcem.20170503.15
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 93
EP  - 96
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170503.15
AB  - The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).
VL  - 5
IS  - 3
ER  -

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Author Information

Chitanava Tamara

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Egiev Ivan

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Chechelian Valerii

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Dzhopua Maksim

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Trofimenko Artem

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Zanin Sergey

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Turovaya Alla

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Kade Azamat

Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia

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APA Style

Chitanava Tamara, Egiev Ivan, Chechelian Valerii, Dzhopua Maksim, Trofimenko Artem, et al. (2017). The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. American Journal of Clinical and Experimental Medicine, 5(3), 93-96. https://doi.org/10.11648/j.ajcem.20170503.15

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ACS Style

Chitanava Tamara; Egiev Ivan; Chechelian Valerii; Dzhopua Maksim; Trofimenko Artem, et al. The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. Am. J. Clin. Exp. Med. 2017, 5(3), 93-96. doi: 10.11648/j.ajcem.20170503.15

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AMA Style

Chitanava Tamara, Egiev Ivan, Chechelian Valerii, Dzhopua Maksim, Trofimenko Artem, et al. The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. Am J Clin Exp Med. 2017;5(3):93-96. doi: 10.11648/j.ajcem.20170503.15

Copy | Download

@article{10.11648/j.ajcem.20170503.15,
  author = {Chitanava Tamara and Egiev Ivan and Chechelian Valerii and Dzhopua Maksim and Trofimenko Artem and Zanin Sergey and Turovaya Alla and Kade Azamat},
  title = {The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {3},
  pages = {93-96},
  doi = {10.11648/j.ajcem.20170503.15},
  url = {https://doi.org/10.11648/j.ajcem.20170503.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170503.15},
  abstract = {The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke
AU  - Chitanava Tamara
AU  - Egiev Ivan
AU  - Chechelian Valerii
AU  - Dzhopua Maksim
AU  - Trofimenko Artem
AU  - Zanin Sergey
AU  - Turovaya Alla
AU  - Kade Azamat
Y1  - 2017/05/09
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170503.15
DO  - 10.11648/j.ajcem.20170503.15
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 93
EP  - 96
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170503.15
AB  - The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).
VL  - 5
IS  - 3
ER  -

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