Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering

Md. Shariful Islam; Mitsugu Todo

doi:doi:10.11648/j.ajcem.20150305.22

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Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering

Md. Shariful Islam, Mitsugu Todo

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 5)

Received: 12 November 2015 Published: 14 November 2015

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Abstract

Highly porous HA scaffolds were synthesized from bioceramics by using the polyurethane (PU) sponge template method. The as-prepared HA scaffolds were then fabricated with poly (vinyl alcohol)/chitosan (PVA/CS) and collagen/chitosan (COL/CS) polymeric materials at 4:1 ratio in coating and 2-phase atmospheric condition. Further, the porous microstructure of fabricated biomaterials were characterized by FE-SEM and mechanical properties were tested by using Shimadzu Compact Tabletop Testing Machine EZTest. It was revealed from the study that incorporation of PVA/CS or COL/CS polymeric materials into pure HA scaffolds either coating or 2-phase condition enhanced the mechanical properties of fabricated biomaterials significantly. Biocompatibility of fabricated biomaterials were checked by culturing Human Mesenchymal Stem cell (hMSC) for a period of 7 days over the prepared scaffolds and it was found that hMSC responded well and grown excellently over the all specimens of fabricated scaffolds. Finally, the results revealed that maximum stress value (0.77 MPa) was obtained from HA-PVA/CS 2-phase with cell samples of 7 days culture and followed by HA-PVA/CS coating with cell (0.75 MPa) due to formation of extra cellular matrix (ECM) reinforcement which allowed specimens undergo densification and stress continued to increase.

Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 5)
DOI	10.11648/j.ajcem.20150305.22
Page(s)	268-274
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Porous Scaffolds, Mechanical Properties, Biocompatibility, Tissue Engineering, Human Mesenchymal Stem cell (hMSC)

References

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APA Style

Md. Shariful Islam, Mitsugu Todo. (2015). Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering. American Journal of Clinical and Experimental Medicine, 3(5), 268-274. https://doi.org/10.11648/j.ajcem.20150305.22

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ACS Style

Md. Shariful Islam; Mitsugu Todo. Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering. Am. J. Clin. Exp. Med. 2015, 3(5), 268-274. doi: 10.11648/j.ajcem.20150305.22

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AMA Style

Md. Shariful Islam, Mitsugu Todo. Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering. Am J Clin Exp Med. 2015;3(5):268-274. doi: 10.11648/j.ajcem.20150305.22

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@article{10.11648/j.ajcem.20150305.22,
  author = {Md. Shariful Islam and Mitsugu Todo},
  title = {Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {5},
  pages = {268-274},
  doi = {10.11648/j.ajcem.20150305.22},
  url = {https://doi.org/10.11648/j.ajcem.20150305.22},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150305.22},
  abstract = {Highly porous HA scaffolds were synthesized from bioceramics by using the polyurethane (PU) sponge template method. The as-prepared HA scaffolds were then fabricated with poly (vinyl alcohol)/chitosan (PVA/CS) and collagen/chitosan (COL/CS) polymeric materials at 4:1 ratio in coating and 2-phase atmospheric condition. Further, the porous microstructure of fabricated biomaterials were characterized by FE-SEM and mechanical properties were tested by using Shimadzu Compact Tabletop Testing Machine EZTest. It was revealed from the study that incorporation of PVA/CS or COL/CS polymeric materials into pure HA scaffolds either coating or 2-phase condition enhanced the mechanical properties of fabricated biomaterials significantly. Biocompatibility of fabricated biomaterials were checked by culturing Human Mesenchymal Stem cell (hMSC) for a period of 7 days over the prepared scaffolds and it was found that hMSC responded well and grown excellently over the all specimens of fabricated scaffolds. Finally, the results revealed that maximum stress value (0.77 MPa) was obtained from HA-PVA/CS 2-phase with cell samples of 7 days culture and followed by HA-PVA/CS coating with cell (0.75 MPa) due to formation of extra cellular matrix (ECM) reinforcement which allowed specimens undergo densification and stress continued to increase.},
 year = {2015}
}

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TY  - JOUR
T1  - Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering
AU  - Md. Shariful Islam
AU  - Mitsugu Todo
Y1  - 2015/11/14
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150305.22
DO  - 10.11648/j.ajcem.20150305.22
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 268
EP  - 274
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150305.22
AB  - Highly porous HA scaffolds were synthesized from bioceramics by using the polyurethane (PU) sponge template method. The as-prepared HA scaffolds were then fabricated with poly (vinyl alcohol)/chitosan (PVA/CS) and collagen/chitosan (COL/CS) polymeric materials at 4:1 ratio in coating and 2-phase atmospheric condition. Further, the porous microstructure of fabricated biomaterials were characterized by FE-SEM and mechanical properties were tested by using Shimadzu Compact Tabletop Testing Machine EZTest. It was revealed from the study that incorporation of PVA/CS or COL/CS polymeric materials into pure HA scaffolds either coating or 2-phase condition enhanced the mechanical properties of fabricated biomaterials significantly. Biocompatibility of fabricated biomaterials were checked by culturing Human Mesenchymal Stem cell (hMSC) for a period of 7 days over the prepared scaffolds and it was found that hMSC responded well and grown excellently over the all specimens of fabricated scaffolds. Finally, the results revealed that maximum stress value (0.77 MPa) was obtained from HA-PVA/CS 2-phase with cell samples of 7 days culture and followed by HA-PVA/CS coating with cell (0.75 MPa) due to formation of extra cellular matrix (ECM) reinforcement which allowed specimens undergo densification and stress continued to increase.
VL  - 3
IS  - 5
ER  -

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Author Information

Md. Shariful Islam

Research Institute for Applied Mechanics, Kyushu University, Fukuoka, Japan
Mitsugu Todo

Research Institute for Applied Mechanics, Kyushu University, Fukuoka, Japan

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APA Style

Md. Shariful Islam, Mitsugu Todo. (2015). Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering. American Journal of Clinical and Experimental Medicine, 3(5), 268-274. https://doi.org/10.11648/j.ajcem.20150305.22

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ACS Style

Md. Shariful Islam; Mitsugu Todo. Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering. Am. J. Clin. Exp. Med. 2015, 3(5), 268-274. doi: 10.11648/j.ajcem.20150305.22

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AMA Style

Md. Shariful Islam, Mitsugu Todo. Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering. Am J Clin Exp Med. 2015;3(5):268-274. doi: 10.11648/j.ajcem.20150305.22

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@article{10.11648/j.ajcem.20150305.22,
  author = {Md. Shariful Islam and Mitsugu Todo},
  title = {Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {5},
  pages = {268-274},
  doi = {10.11648/j.ajcem.20150305.22},
  url = {https://doi.org/10.11648/j.ajcem.20150305.22},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150305.22},
  abstract = {Highly porous HA scaffolds were synthesized from bioceramics by using the polyurethane (PU) sponge template method. The as-prepared HA scaffolds were then fabricated with poly (vinyl alcohol)/chitosan (PVA/CS) and collagen/chitosan (COL/CS) polymeric materials at 4:1 ratio in coating and 2-phase atmospheric condition. Further, the porous microstructure of fabricated biomaterials were characterized by FE-SEM and mechanical properties were tested by using Shimadzu Compact Tabletop Testing Machine EZTest. It was revealed from the study that incorporation of PVA/CS or COL/CS polymeric materials into pure HA scaffolds either coating or 2-phase condition enhanced the mechanical properties of fabricated biomaterials significantly. Biocompatibility of fabricated biomaterials were checked by culturing Human Mesenchymal Stem cell (hMSC) for a period of 7 days over the prepared scaffolds and it was found that hMSC responded well and grown excellently over the all specimens of fabricated scaffolds. Finally, the results revealed that maximum stress value (0.77 MPa) was obtained from HA-PVA/CS 2-phase with cell samples of 7 days culture and followed by HA-PVA/CS coating with cell (0.75 MPa) due to formation of extra cellular matrix (ECM) reinforcement which allowed specimens undergo densification and stress continued to increase.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering
AU  - Md. Shariful Islam
AU  - Mitsugu Todo
Y1  - 2015/11/14
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150305.22
DO  - 10.11648/j.ajcem.20150305.22
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 268
EP  - 274
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150305.22
AB  - Highly porous HA scaffolds were synthesized from bioceramics by using the polyurethane (PU) sponge template method. The as-prepared HA scaffolds were then fabricated with poly (vinyl alcohol)/chitosan (PVA/CS) and collagen/chitosan (COL/CS) polymeric materials at 4:1 ratio in coating and 2-phase atmospheric condition. Further, the porous microstructure of fabricated biomaterials were characterized by FE-SEM and mechanical properties were tested by using Shimadzu Compact Tabletop Testing Machine EZTest. It was revealed from the study that incorporation of PVA/CS or COL/CS polymeric materials into pure HA scaffolds either coating or 2-phase condition enhanced the mechanical properties of fabricated biomaterials significantly. Biocompatibility of fabricated biomaterials were checked by culturing Human Mesenchymal Stem cell (hMSC) for a period of 7 days over the prepared scaffolds and it was found that hMSC responded well and grown excellently over the all specimens of fabricated scaffolds. Finally, the results revealed that maximum stress value (0.77 MPa) was obtained from HA-PVA/CS 2-phase with cell samples of 7 days culture and followed by HA-PVA/CS coating with cell (0.75 MPa) due to formation of extra cellular matrix (ECM) reinforcement which allowed specimens undergo densification and stress continued to increase.
VL  - 3
IS  - 5
ER  -

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