ackground: Breast cancer is a type of cancer that has a high prevalence, can occur in men and women. Cytokines are factors that are known to have good properties of tumor growth and tumor inhibitory effects. Cytokine has an important role in the control of the immune system. Development of breast carcinoma is closely related to mRNA expression of IL-6 as pro-inflammatory cytokine and mRNA expression of IL-10 as anti-inflammatory cytokine. Purpose: Examine changes in mRNA expression of proinflammatory cytokine and anti-inflammatory cytokine in breast cancer patients with or without chemotherapy. Method: This is a case-control study, 12 subjects with breast cancer with chemotherapy as case group and 12 subjects with breast cancer without chemotherapy as control group. In this study we using PCR and RFLP techniques to detect polymorphisms in IL-6 and IL-10 gene. Results: In RFLP analysis, found that IL-6 and IL-10 gene were not polymorphic in all subjects for both case and control groups. Mean value of IL-6 mRNA on breast cancer patients without receiving chemotheraphy was 10.84±0.48 whereas on breast cancer patients with chemotherapy was 6.28±1.19. Mean value of IL-10 mRNA on breast cancer patients without receiving chemotheraphy was 6.76±0.81 whereas on breast cancer patients with chemotherapy was 9.81±1.07. The mean value of IL-6 mRNA on breast cancer patients without receiving chemotheraphy was statistically significant higher than in patients with chemotheraphy. In contrast, mean value of IL-10 mRNA on breast cancer patients without receiving chemotheraphy was statistically significant lower than in patients with chemotherapy. Conclusion: Combination of chemotherapy docetaxel, adriamycin, cyclophosphamide cause a decrease in mRNA expression of IL-6 and an increase in the expression of mRNA of IL-10 in patients with breast cancer.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 3, Issue 3) |
| DOI | 10.11648/j.ajcem.20150303.15 |
| Page(s) | 99-104 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Breast Cancer, Chemotherapy, mRNA IL-6, IL-10
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APA Style
Victor Pontoh, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta. (2015). Profile of mRNA Expression of IL-6 and IL-10 in Breast Cancer Patients with or Without Chemotherapy. American Journal of Clinical and Experimental Medicine, 3(3), 99-104. https://doi.org/10.11648/j.ajcem.20150303.15
ACS Style
Victor Pontoh; Daniel Sampepajung; Andi Asadul Islam; Mochammad Hatta. Profile of mRNA Expression of IL-6 and IL-10 in Breast Cancer Patients with or Without Chemotherapy. Am. J. Clin. Exp. Med. 2015, 3(3), 99-104. doi: 10.11648/j.ajcem.20150303.15
AMA Style
Victor Pontoh, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta. Profile of mRNA Expression of IL-6 and IL-10 in Breast Cancer Patients with or Without Chemotherapy. Am J Clin Exp Med. 2015;3(3):99-104. doi: 10.11648/j.ajcem.20150303.15
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Download@article{10.11648/j.ajcem.20150303.15,
author = {Victor Pontoh and Daniel Sampepajung and Andi Asadul Islam and Mochammad Hatta},
title = {Profile of mRNA Expression of IL-6 and IL-10 in Breast Cancer Patients with or Without Chemotherapy},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {3},
number = {3},
pages = {99-104},
doi = {10.11648/j.ajcem.20150303.15},
url = {https://doi.org/10.11648/j.ajcem.20150303.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150303.15},
abstract = {ackground: Breast cancer is a type of cancer that has a high prevalence, can occur in men and women. Cytokines are factors that are known to have good properties of tumor growth and tumor inhibitory effects. Cytokine has an important role in the control of the immune system. Development of breast carcinoma is closely related to mRNA expression of IL-6 as pro-inflammatory cytokine and mRNA expression of IL-10 as anti-inflammatory cytokine. Purpose: Examine changes in mRNA expression of proinflammatory cytokine and anti-inflammatory cytokine in breast cancer patients with or without chemotherapy. Method: This is a case-control study, 12 subjects with breast cancer with chemotherapy as case group and 12 subjects with breast cancer without chemotherapy as control group. In this study we using PCR and RFLP techniques to detect polymorphisms in IL-6 and IL-10 gene. Results: In RFLP analysis, found that IL-6 and IL-10 gene were not polymorphic in all subjects for both case and control groups. Mean value of IL-6 mRNA on breast cancer patients without receiving chemotheraphy was 10.84±0.48 whereas on breast cancer patients with chemotherapy was 6.28±1.19. Mean value of IL-10 mRNA on breast cancer patients without receiving chemotheraphy was 6.76±0.81 whereas on breast cancer patients with chemotherapy was 9.81±1.07. The mean value of IL-6 mRNA on breast cancer patients without receiving chemotheraphy was statistically significant higher than in patients with chemotheraphy. In contrast, mean value of IL-10 mRNA on breast cancer patients without receiving chemotheraphy was statistically significant lower than in patients with chemotherapy. Conclusion: Combination of chemotherapy docetaxel, adriamycin, cyclophosphamide cause a decrease in mRNA expression of IL-6 and an increase in the expression of mRNA of IL-10 in patients with breast cancer.},
year = {2015}
}
TY - JOUR T1 - Profile of mRNA Expression of IL-6 and IL-10 in Breast Cancer Patients with or Without Chemotherapy AU - Victor Pontoh AU - Daniel Sampepajung AU - Andi Asadul Islam AU - Mochammad Hatta Y1 - 2015/05/11 PY - 2015 N1 - https://doi.org/10.11648/j.ajcem.20150303.15 DO - 10.11648/j.ajcem.20150303.15 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 99 EP - 104 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20150303.15 AB - ackground: Breast cancer is a type of cancer that has a high prevalence, can occur in men and women. Cytokines are factors that are known to have good properties of tumor growth and tumor inhibitory effects. Cytokine has an important role in the control of the immune system. Development of breast carcinoma is closely related to mRNA expression of IL-6 as pro-inflammatory cytokine and mRNA expression of IL-10 as anti-inflammatory cytokine. Purpose: Examine changes in mRNA expression of proinflammatory cytokine and anti-inflammatory cytokine in breast cancer patients with or without chemotherapy. Method: This is a case-control study, 12 subjects with breast cancer with chemotherapy as case group and 12 subjects with breast cancer without chemotherapy as control group. In this study we using PCR and RFLP techniques to detect polymorphisms in IL-6 and IL-10 gene. Results: In RFLP analysis, found that IL-6 and IL-10 gene were not polymorphic in all subjects for both case and control groups. Mean value of IL-6 mRNA on breast cancer patients without receiving chemotheraphy was 10.84±0.48 whereas on breast cancer patients with chemotherapy was 6.28±1.19. Mean value of IL-10 mRNA on breast cancer patients without receiving chemotheraphy was 6.76±0.81 whereas on breast cancer patients with chemotherapy was 9.81±1.07. The mean value of IL-6 mRNA on breast cancer patients without receiving chemotheraphy was statistically significant higher than in patients with chemotheraphy. In contrast, mean value of IL-10 mRNA on breast cancer patients without receiving chemotheraphy was statistically significant lower than in patients with chemotherapy. Conclusion: Combination of chemotherapy docetaxel, adriamycin, cyclophosphamide cause a decrease in mRNA expression of IL-6 and an increase in the expression of mRNA of IL-10 in patients with breast cancer. VL - 3 IS - 3 ER -
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