Green Synthesis of Silver Nanoparticles Using <i>Euphorbia hirta</i> Leaf Extract and the Determination of Their Antimicrobial Activity

Egbunu Iganya Edith; Philip Felix Uzor

doi:doi:10.11648/j.abb.20210904.14

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Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity

Egbunu Iganya Edith, Philip Felix Uzor

Published in Advances in Bioscience and Bioengineering (Volume 9, Issue 4)

Received: 25 November 2021 Accepted: 14 December 2021 Published: 24 December 2021

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Abstract

Nanotechnology is a fast-growing field of science. Nanoparticles get much attention due to their unique physicochemical, optical and thermal activities. Silver nanoparticles have been used in experiments to treat infectious diseases. The goal of the research was to make silver nanoparticles using Euphorbia hirta extract, physically characterize the nanoparticles obtained and, to evaluate silver nanoparticles' antibacterial properties. The leaf extract of E. hirta (asthma weed) was used for the reduction of 1 mM silver nitrate (AgNO₃) solution to silver nanoparticles (SNPs). SNPs were made by combining 50 mL of aqueous plant extract with 250 mL of AgNO₃ solution to make SNPs. The mixture was monitored for two hours. The synthesized SNPs were characterized by UV-Vis, FTIR spectroscopy and particle size. The antimicrobial activity of the SNPs was tested against Escherichia. coli, Pseudomonas. aeruginosa, Salmonella. typhi, Bacillus. subtilis and Candida. albicans. The reaction medium's hue shifted from yellow to brown. The results of the UV-vis analysis of the particles showed that at 430 nm the particles had the maximum absorption (λ max) within 2 hours. The FTIR identified carboxylic acid and other functional groups. The polydispersity index (PDI) and Z-average particle size were found to be 0.426 and 274 nm, respectively. The results of the antimicrobial studies showed sufficient growth inhibition of the bacteria by the SNPs the minimum inhibitory concentration (MIC) ranging from 7 µg-10 µg. It was concluded that SNPs were synthesized using E. hirta leaf extract. The synthesized SNPs possess good activity against pathogenic microorganisms.

Published in	Advances in Bioscience and Bioengineering (Volume 9, Issue 4)
DOI	10.11648/j.abb.20210904.14
Page(s)	124-129
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Nanoparticles, Synthesis, Extraction, Antimicrobial, Nanotechnology

References

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APA Style

Egbunu Iganya Edith, Philip Felix Uzor. (2021). Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity. Advances in Bioscience and Bioengineering, 9(4), 124-129. https://doi.org/10.11648/j.abb.20210904.14

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ACS Style

Egbunu Iganya Edith; Philip Felix Uzor. Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity. Adv. BioSci. Bioeng. 2021, 9(4), 124-129. doi: 10.11648/j.abb.20210904.14

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Egbunu Iganya Edith, Philip Felix Uzor. Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity. Adv BioSci Bioeng. 2021;9(4):124-129. doi: 10.11648/j.abb.20210904.14

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@article{10.11648/j.abb.20210904.14,
  author = {Egbunu Iganya Edith and Philip Felix Uzor},
  title = {Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {9},
  number = {4},
  pages = {124-129},
  doi = {10.11648/j.abb.20210904.14},
  url = {https://doi.org/10.11648/j.abb.20210904.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210904.14},
  abstract = {Nanotechnology is a fast-growing field of science. Nanoparticles get much attention due to their unique physicochemical, optical and thermal activities. Silver nanoparticles have been used in experiments to treat infectious diseases. The goal of the research was to make silver nanoparticles using Euphorbia hirta extract, physically characterize the nanoparticles obtained and, to evaluate silver nanoparticles' antibacterial properties. The leaf extract of E. hirta (asthma weed) was used for the reduction of 1 mM silver nitrate (AgNO3) solution to silver nanoparticles (SNPs). SNPs were made by combining 50 mL of aqueous plant extract with 250 mL of AgNO3 solution to make SNPs. The mixture was monitored for two hours. The synthesized SNPs were characterized by UV-Vis, FTIR spectroscopy and particle size. The antimicrobial activity of the SNPs was tested against Escherichia. coli, Pseudomonas. aeruginosa, Salmonella. typhi, Bacillus. subtilis and Candida. albicans. The reaction medium's hue shifted from yellow to brown. The results of the UV-vis analysis of the particles showed that at 430 nm the particles had the maximum absorption (λ max) within 2 hours. The FTIR identified carboxylic acid and other functional groups. The polydispersity index (PDI) and Z-average particle size were found to be 0.426 and 274 nm, respectively. The results of the antimicrobial studies showed sufficient growth inhibition of the bacteria by the SNPs the minimum inhibitory concentration (MIC) ranging from 7 µg-10 µg. It was concluded that SNPs were synthesized using E. hirta leaf extract. The synthesized SNPs possess good activity against pathogenic microorganisms.},
 year = {2021}
}

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TY - JOUR
T1 - Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity
AU - Egbunu Iganya Edith
AU - Philip Felix Uzor
Y1 - 2021/12/24
PY - 2021
N1 - https://doi.org/10.11648/j.abb.20210904.14
DO - 10.11648/j.abb.20210904.14
T2 - Advances in Bioscience and Bioengineering
JF - Advances in Bioscience and Bioengineering
JO - Advances in Bioscience and Bioengineering
SP - 124
EP - 129
PB - Science Publishing Group
SN - 2330-4162
UR - https://doi.org/10.11648/j.abb.20210904.14
AB - Nanotechnology is a fast-growing field of science. Nanoparticles get much attention due to their unique physicochemical, optical and thermal activities. Silver nanoparticles have been used in experiments to treat infectious diseases. The goal of the research was to make silver nanoparticles using Euphorbia hirta extract, physically characterize the nanoparticles obtained and, to evaluate silver nanoparticles' antibacterial properties. The leaf extract of E. hirta (asthma weed) was used for the reduction of 1 mM silver nitrate (AgNO3) solution to silver nanoparticles (SNPs). SNPs were made by combining 50 mL of aqueous plant extract with 250 mL of AgNO3 solution to make SNPs. The mixture was monitored for two hours. The synthesized SNPs were characterized by UV-Vis, FTIR spectroscopy and particle size. The antimicrobial activity of the SNPs was tested against Escherichia. coli, Pseudomonas. aeruginosa, Salmonella. typhi, Bacillus. subtilis and Candida. albicans. The reaction medium's hue shifted from yellow to brown. The results of the UV-vis analysis of the particles showed that at 430 nm the particles had the maximum absorption (λ max) within 2 hours. The FTIR identified carboxylic acid and other functional groups. The polydispersity index (PDI) and Z-average particle size were found to be 0.426 and 274 nm, respectively. The results of the antimicrobial studies showed sufficient growth inhibition of the bacteria by the SNPs the minimum inhibitory concentration (MIC) ranging from 7 µg-10 µg. It was concluded that SNPs were synthesized using E. hirta leaf extract. The synthesized SNPs possess good activity against pathogenic microorganisms.
VL - 9
IS - 4
ER -

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Author Information

Egbunu Iganya Edith

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Madonna University, Elele, Nigeria
Philip Felix Uzor

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Madonna University, Elele, Nigeria

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APA Style

Egbunu Iganya Edith, Philip Felix Uzor. (2021). Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity. Advances in Bioscience and Bioengineering, 9(4), 124-129. https://doi.org/10.11648/j.abb.20210904.14

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ACS Style

Egbunu Iganya Edith; Philip Felix Uzor. Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity. Adv. BioSci. Bioeng. 2021, 9(4), 124-129. doi: 10.11648/j.abb.20210904.14

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AMA Style

Egbunu Iganya Edith, Philip Felix Uzor. Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity. Adv BioSci Bioeng. 2021;9(4):124-129. doi: 10.11648/j.abb.20210904.14

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@article{10.11648/j.abb.20210904.14,
  author = {Egbunu Iganya Edith and Philip Felix Uzor},
  title = {Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {9},
  number = {4},
  pages = {124-129},
  doi = {10.11648/j.abb.20210904.14},
  url = {https://doi.org/10.11648/j.abb.20210904.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210904.14},
  abstract = {Nanotechnology is a fast-growing field of science. Nanoparticles get much attention due to their unique physicochemical, optical and thermal activities. Silver nanoparticles have been used in experiments to treat infectious diseases. The goal of the research was to make silver nanoparticles using Euphorbia hirta extract, physically characterize the nanoparticles obtained and, to evaluate silver nanoparticles' antibacterial properties. The leaf extract of E. hirta (asthma weed) was used for the reduction of 1 mM silver nitrate (AgNO3) solution to silver nanoparticles (SNPs). SNPs were made by combining 50 mL of aqueous plant extract with 250 mL of AgNO3 solution to make SNPs. The mixture was monitored for two hours. The synthesized SNPs were characterized by UV-Vis, FTIR spectroscopy and particle size. The antimicrobial activity of the SNPs was tested against Escherichia. coli, Pseudomonas. aeruginosa, Salmonella. typhi, Bacillus. subtilis and Candida. albicans. The reaction medium's hue shifted from yellow to brown. The results of the UV-vis analysis of the particles showed that at 430 nm the particles had the maximum absorption (λ max) within 2 hours. The FTIR identified carboxylic acid and other functional groups. The polydispersity index (PDI) and Z-average particle size were found to be 0.426 and 274 nm, respectively. The results of the antimicrobial studies showed sufficient growth inhibition of the bacteria by the SNPs the minimum inhibitory concentration (MIC) ranging from 7 µg-10 µg. It was concluded that SNPs were synthesized using E. hirta leaf extract. The synthesized SNPs possess good activity against pathogenic microorganisms.},
 year = {2021}
}

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