Maximization of Astaxanthin Production from Green Microalga <i>Haematococcus pluvialis</i> Using Internally-Illuminated Photobioreactor

Yiu Hang Ho; Ho Man Leung; Shuk Ying Yuen; Kei Shing Ng; Tak Sing Li; Lap Ming Yuen; Yee Keung Wong

doi:doi:10.11648/j.abb.20180602.11

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Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor

Yiu Hang Ho, Ho Man Leung, Shuk Ying Yuen, Kei Shing Ng, Tak Sing Li, Lap Ming Yuen, Yee Keung Wong

Published in Advances in Bioscience and Bioengineering (Volume 6, Issue 2)

Received: 31 May 2018 Accepted: 8 June 2018 Published: 5 July 2018

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Abstract

An internally-illuminated photobioreactor was designed to maximize the astaxanthin production by Haematococcus pluvialis. Four optimization steps were conducted: 1. light wavelength 2. light intensity 3. astaxanthin formation and 4. astaxanthin extraction methods. Efficient biomass production of H. pluvialis of 4.58 ± 0.15 × 10⁵ cells/ml and dry biomass of 520 ± 12.5 mg/L was accomplished under red LED light (660 nm) with 70 μmol m^-2 s^-1. Besides, the biomass production can be optimized to 5.31 ± 0.15 × 10⁵ cells/ml and dry biomass of 680 ± 10.5 mg/L under 140 μmol m^-2 s^-1 in the light intensity of 70-210 μmol m-2 s-1. Furthermore, the astaxanthin accumulation was significant with 7 days encystment under 140 μmol m^-2 s^-1 blue LED lights. For extraction method, using hydrochloric acid could obtain the highest astaxanthin yield of 3.85 ± 0.05% (% to dry weight). Further studies were proposed whatever such photobioreactor can be applied to different microalgal strains.

Published in	Advances in Bioscience and Bioengineering (Volume 6, Issue 2)
DOI	10.11648/j.abb.20180602.11
Page(s)	10-22
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Astaxanthin, Haematococcus Pluvialis, Internally-Illuminated Photobioreactor, Lighting, Cell Disruption, Extractability

References

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APA Style

Yiu Hang Ho, Ho Man Leung, Shuk Ying Yuen, Kei Shing Ng, Tak Sing Li, et al. (2018). Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor. Advances in Bioscience and Bioengineering, 6(2), 10-22. https://doi.org/10.11648/j.abb.20180602.11

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ACS Style

Yiu Hang Ho; Ho Man Leung; Shuk Ying Yuen; Kei Shing Ng; Tak Sing Li, et al. Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor. Adv. BioSci. Bioeng. 2018, 6(2), 10-22. doi: 10.11648/j.abb.20180602.11

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AMA Style

Yiu Hang Ho, Ho Man Leung, Shuk Ying Yuen, Kei Shing Ng, Tak Sing Li, et al. Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor. Adv BioSci Bioeng. 2018;6(2):10-22. doi: 10.11648/j.abb.20180602.11

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@article{10.11648/j.abb.20180602.11,
  author = {Yiu Hang Ho and Ho Man Leung and Shuk Ying Yuen and Kei Shing Ng and Tak Sing Li and Lap Ming Yuen and Yee Keung Wong},
  title = {Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {6},
  number = {2},
  pages = {10-22},
  doi = {10.11648/j.abb.20180602.11},
  url = {https://doi.org/10.11648/j.abb.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20180602.11},
  abstract = {An internally-illuminated photobioreactor was designed to maximize the astaxanthin production by Haematococcus pluvialis. Four optimization steps were conducted: 1. light wavelength 2. light intensity 3. astaxanthin formation and 4. astaxanthin extraction methods. Efficient biomass production of H. pluvialis of 4.58 ± 0.15 × 105 cells/ml and dry biomass of 520 ± 12.5 mg/L was accomplished under red LED light (660 nm) with 70 μmol m-2 s-1. Besides, the biomass production can be optimized to 5.31 ± 0.15 × 105 cells/ml and dry biomass of 680 ± 10.5 mg/L under 140 μmol m-2 s-1 in the light intensity of 70-210 μmol m-2 s-1. Furthermore, the astaxanthin accumulation was significant with 7 days encystment under 140 μmol m-2 s-1 blue LED lights. For extraction method, using hydrochloric acid could obtain the highest astaxanthin yield of 3.85 ± 0.05% (% to dry weight). Further studies were proposed whatever such photobioreactor can be applied to different microalgal strains.},
 year = {2018}
}

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TY  - JOUR
T1  - Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor
AU  - Yiu Hang Ho
AU  - Ho Man Leung
AU  - Shuk Ying Yuen
AU  - Kei Shing Ng
AU  - Tak Sing Li
AU  - Lap Ming Yuen
AU  - Yee Keung Wong
Y1  - 2018/07/05
PY  - 2018
N1  - https://doi.org/10.11648/j.abb.20180602.11
DO  - 10.11648/j.abb.20180602.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 10
EP  - 22
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20180602.11
AB  - An internally-illuminated photobioreactor was designed to maximize the astaxanthin production by Haematococcus pluvialis. Four optimization steps were conducted: 1. light wavelength 2. light intensity 3. astaxanthin formation and 4. astaxanthin extraction methods. Efficient biomass production of H. pluvialis of 4.58 ± 0.15 × 105 cells/ml and dry biomass of 520 ± 12.5 mg/L was accomplished under red LED light (660 nm) with 70 μmol m-2 s-1. Besides, the biomass production can be optimized to 5.31 ± 0.15 × 105 cells/ml and dry biomass of 680 ± 10.5 mg/L under 140 μmol m-2 s-1 in the light intensity of 70-210 μmol m-2 s-1. Furthermore, the astaxanthin accumulation was significant with 7 days encystment under 140 μmol m-2 s-1 blue LED lights. For extraction method, using hydrochloric acid could obtain the highest astaxanthin yield of 3.85 ± 0.05% (% to dry weight). Further studies were proposed whatever such photobioreactor can be applied to different microalgal strains.
VL  - 6
IS  - 2
ER  -

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Author Information

Yiu Hang Ho

School of Science and Technology, the Open University of Hong Kong, HKSAR, China
Ho Man Leung

Department of Biology, Hong Kong Baptist University, HKSAR, China
Shuk Ying Yuen

School of Science and Technology, the Open University of Hong Kong, HKSAR, China
Kei Shing Ng

School of Science and Technology, the Open University of Hong Kong, HKSAR, China
Tak Sing Li

Institute for Research in Innovative Technology & Sustainability (IRITS), the Open University of Hong Kong, HKSAR, China
Lap Ming Yuen

School of Science and Technology, the Open University of Hong Kong, HKSAR, China
Yee Keung Wong

Institute for Research in Innovative Technology & Sustainability (IRITS), the Open University of Hong Kong, HKSAR, China

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Yiu Hang Ho, Ho Man Leung, Shuk Ying Yuen, Kei Shing Ng, Tak Sing Li, et al. (2018). Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor. Advances in Bioscience and Bioengineering, 6(2), 10-22. https://doi.org/10.11648/j.abb.20180602.11

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ACS Style

Yiu Hang Ho; Ho Man Leung; Shuk Ying Yuen; Kei Shing Ng; Tak Sing Li, et al. Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor. Adv. BioSci. Bioeng. 2018, 6(2), 10-22. doi: 10.11648/j.abb.20180602.11

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AMA Style

Yiu Hang Ho, Ho Man Leung, Shuk Ying Yuen, Kei Shing Ng, Tak Sing Li, et al. Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor. Adv BioSci Bioeng. 2018;6(2):10-22. doi: 10.11648/j.abb.20180602.11

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@article{10.11648/j.abb.20180602.11,
  author = {Yiu Hang Ho and Ho Man Leung and Shuk Ying Yuen and Kei Shing Ng and Tak Sing Li and Lap Ming Yuen and Yee Keung Wong},
  title = {Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {6},
  number = {2},
  pages = {10-22},
  doi = {10.11648/j.abb.20180602.11},
  url = {https://doi.org/10.11648/j.abb.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20180602.11},
  abstract = {An internally-illuminated photobioreactor was designed to maximize the astaxanthin production by Haematococcus pluvialis. Four optimization steps were conducted: 1. light wavelength 2. light intensity 3. astaxanthin formation and 4. astaxanthin extraction methods. Efficient biomass production of H. pluvialis of 4.58 ± 0.15 × 105 cells/ml and dry biomass of 520 ± 12.5 mg/L was accomplished under red LED light (660 nm) with 70 μmol m-2 s-1. Besides, the biomass production can be optimized to 5.31 ± 0.15 × 105 cells/ml and dry biomass of 680 ± 10.5 mg/L under 140 μmol m-2 s-1 in the light intensity of 70-210 μmol m-2 s-1. Furthermore, the astaxanthin accumulation was significant with 7 days encystment under 140 μmol m-2 s-1 blue LED lights. For extraction method, using hydrochloric acid could obtain the highest astaxanthin yield of 3.85 ± 0.05% (% to dry weight). Further studies were proposed whatever such photobioreactor can be applied to different microalgal strains.},
 year = {2018}
}

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TY  - JOUR
T1  - Maximization of Astaxanthin Production from Green Microalga Haematococcus pluvialis Using Internally-Illuminated Photobioreactor
AU  - Yiu Hang Ho
AU  - Ho Man Leung
AU  - Shuk Ying Yuen
AU  - Kei Shing Ng
AU  - Tak Sing Li
AU  - Lap Ming Yuen
AU  - Yee Keung Wong
Y1  - 2018/07/05
PY  - 2018
N1  - https://doi.org/10.11648/j.abb.20180602.11
DO  - 10.11648/j.abb.20180602.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 10
EP  - 22
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20180602.11
AB  - An internally-illuminated photobioreactor was designed to maximize the astaxanthin production by Haematococcus pluvialis. Four optimization steps were conducted: 1. light wavelength 2. light intensity 3. astaxanthin formation and 4. astaxanthin extraction methods. Efficient biomass production of H. pluvialis of 4.58 ± 0.15 × 105 cells/ml and dry biomass of 520 ± 12.5 mg/L was accomplished under red LED light (660 nm) with 70 μmol m-2 s-1. Besides, the biomass production can be optimized to 5.31 ± 0.15 × 105 cells/ml and dry biomass of 680 ± 10.5 mg/L under 140 μmol m-2 s-1 in the light intensity of 70-210 μmol m-2 s-1. Furthermore, the astaxanthin accumulation was significant with 7 days encystment under 140 μmol m-2 s-1 blue LED lights. For extraction method, using hydrochloric acid could obtain the highest astaxanthin yield of 3.85 ± 0.05% (% to dry weight). Further studies were proposed whatever such photobioreactor can be applied to different microalgal strains.
VL  - 6
IS  - 2
ER  -

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